All molecules consist of chemical bonds, and much can be learned from mapping the spatiotemporal dynamics of these bonds. Since its invention a decade ago, stimulated Raman scattering (SRS) microscopy has become a powerful modality for imaging chemical bonds with high sensitivity, resolution, speed and specificity. We introduce the fundamentals of SRS microscopy and review innovations in SRS microscopes and imaging probes. We highlight examples of exciting biological applications, and share our vision for potential future breakthroughs for this technology.
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We thank L. Wei, L. Shi, H. Xiong and X. Liu for reading the manuscript. W.M. acknowledges support from National Institutes of Health (NIH) Director’s New Innovator Award, NIH R01 (EB020892 to W. M.), NIH R01 (GM128214 to W. M.), NIH R01 (GM132860 to W. M.), the Alfred P. Sloan Foundation, the Camille and Henry Dreyfus Foundation, and a Pilot and Feasibility grant from the New York Obesity Nutrition Research Center. F.H. acknowledges support from a Raymond and Beverly Sackler Center Postdoctoral Fellowship.
The authors declare no competing interests.
Peer review information: Rita Strack was the primary editor on this article and managed its editorial process and peer review in collaboration with the rest of the editorial team.
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Hu, F., Shi, L. & Min, W. Biological imaging of chemical bonds by stimulated Raman scattering microscopy. Nat Methods 16, 830–842 (2019). https://doi.org/10.1038/s41592-019-0538-0
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