Fig. 1: Simultaneous control of protein and crRNA expression from Pol II promoters. | Nature Methods

Fig. 1: Simultaneous control of protein and crRNA expression from Pol II promoters.

From: Multiplexed genome engineering by Cas12a and CRISPR arrays encoded on single transcripts

Fig. 1

a, Schematic of a single transcript architecture containing both a protein-coding sequence and a CRISPR array. The transcript encodes for: Protein/Cas12a is a gray/yellow rectangle; Triplex, a tertiary RNA structural motif is a small purple rectangle; DR is a gray square; spacer is a blue square and poly(A) is a red rectangle. b, Validation of Triplex-mediated mRNA stabilization for concomitant gene editing and protein expression. Combinations of crRNA expression constructs and Cas12a proteins used (conditions I–VI). Representative EGFP fluorescent images after cotransfection of different plasmid combinations (conditions I–VI). Scale bar, 1.00 mm. Quantification of gene editing efficiencies (conditions I–VI). Values represent mean ± s.e.m., n = 3 independent experiments.

Back to article page