Fig. 1: Concept, design and implementation of the LADL system. | Nature Methods

Fig. 1: Concept, design and implementation of the LADL system.

From: LADL: light-activated dynamic looping for endogenous gene expression control

Fig. 1

a, Schematic of the LADL system. b,c, Schematic of plasmid constructs encoding the puromycin-selectable LADL anchor and empty anchor control (b) and the LADL ‘bridge + target’, the empty target control and the empty bridge control (c). d, Schematic timeline of seeding, transfection, puromycin selection and blue light illumination of v.6.5 mouse ES cells. e, RT–qPCR analysis of Oct4, Nestin, Nanog, Sox2, dCas9 and CRY2 transcript levels in cotransfected mouse ES cells 36 h after puromycin selection. Data from two independent experiments are shown as replicates 1 (Rep1) and 2 (Rep2), respectively. BL, blue light. f, Immunofluorescence staining for Oct4 in mouse ES cells cotransfected with the indicated plasmids. Scale bars, 50 µm. Images are representative of three independent experiments.

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