Supplementary Figure 6: Simulation of the oblique Gaussian and Bessel light sheet. | Nature Methods

Supplementary Figure 6: Simulation of the oblique Gaussian and Bessel light sheet.

From: Epi-illumination SPIM for volumetric imaging with high spatial-temporal resolution

Supplementary Figure 6

a,b, The simulated intensity maps of the oblique Gaussian and Bessel light sheet within the x'z' plane. Insets of a and b show the corresponding pupil plane, with the red circles indicating the full extent of the pupil of the objective, and the white lines the effective illumination laser pattern (with a uniform intensity) at the pupil plane. The pupil function to generate the Gaussian light sheet comprises light with incident angles ranging from 47.28° to 72.72° (i.e., 60° ± 12.72°). The pupil function to generate the Bessel light sheet is segmented into two parts, one with incident light from 47.28° to 52.72°, the other from 67.28° to 72.72°. c,d, The simulated cross-section profiles along the z'- and x'-axes crossing the peak intensities of the intensity maps in a and b. The FWHMs (of the central peak) along the z'-axis are 730 nm and 554 nm for the Gaussian and Bessel beam correspondingly, while the FWHMs along the x'-axis are 12.8 μm and 18.6 μm.

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