Supplementary Figure 1: Optical-setup layout of the eSPIM system. | Nature Methods

Supplementary Figure 1: Optical-setup layout of the eSPIM system.

From: Epi-illumination SPIM for volumetric imaging with high spatial-temporal resolution

Supplementary Figure 1

O1-O3: objective lenses; M1-M8: mirrors; L1-L9: achromatic plano-concave lenses; CL1-CL3: cylindrical lenses; DM1-DM3: dichroic mirrors; TS1-TS5: translation stages; AOM: acoustic-optical modulator; EF: emission filter. Focal lengths of all lenses: 200 mm (L1), 75 mm (L2), 125 mm (L3), 150 mm (L4), 125 mm (L5), 100 mm (L6), 150 mm (L7), 45 mm (L8), 100 mm (L1), 100 mm (CL1), 200 mm (CL2) and 50 mm (CL3). The diameter of the pinhole is 30 µm. In the case of the Bessel light-sheet, the slit is replaced with a photomask.

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