Supplementary Figure 12: Fast volumetric imaging using the eSPIM system. | Nature Methods

Supplementary Figure 12: Fast volumetric imaging using the eSPIM system.

From: Epi-illumination SPIM for volumetric imaging with high spatial-temporal resolution

Supplementary Figure 12

A Drosophila S2 cell with lysosomes labeled with LysoTracker Deep Red dye. Four maximum intensity projection images at different time points are shown. The triangles highlight two noticeable events. Images were acquired at 15 volumes per second (volume size 35 µm × 35 µm × 7 µm, 34 slices per volume and camera running at 500 frames per second). At this imaging speed, we could reliably perform 3D tracking of lysosome movement dynamics. See also Supplementary Movies 810. Three experiments were repeated independently, with similar results.

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