a,b, Cells are transfected with EGFP-α-tubulin (a) and mRuby2-TOMM20-N-10 (b). The maximum intensity projection (MIP) in the x–y plane is shown together with two representative x–z and y–z slices along the dashed lines. The regions outlined by the dashed boxes are also shown as magnified images. The magnified region in b is shown for four different time points; images are taken at 1.6 s/volume. The white triangles indicate the place where the cell undergoes structural changes. Three experiments were repeated independently, with similar results.