Supplementary Figure 9: Volumetric imaging of over-expressed live HeLa cells. | Nature Methods

Supplementary Figure 9: Volumetric imaging of over-expressed live HeLa cells.

From: Epi-illumination SPIM for volumetric imaging with high spatial-temporal resolution

Supplementary Figure 9

a,b, Cells are transfected with EGFP-α-tubulin (a) and mRuby2-TOMM20-N-10 (b). The maximum intensity projection (MIP) in the xy plane is shown together with two representative xz and yz slices along the dashed lines. The regions outlined by the dashed boxes are also shown as magnified images. The magnified region in b is shown for four different time points; images are taken at 1.6 s/volume. The white triangles indicate the place where the cell undergoes structural changes. Three experiments were repeated independently, with similar results.

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