Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

MICROSCOPY

Peering into cells at high resolution just got easier

Light-sheet fluorescence microscopes are making it possible to follow subcellular dynamics with unprecedented detail, but they are complex to build and maintain. A new method that can generate arbitrary light sheets provides much-needed simplicity and additional versatility.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Fig. 1: Field synthesis can generate arbitrary light sheets, including dithered lattice light sheets.

Katie Vicari and Kim Caesar/Springer Nature

References

  1. Gao, L. Opt. Express 23, 6102–6111 (2015).

    Article  Google Scholar 

  2. Fahrbach, F. O., Simon, P. & Rohrbach, A. Nat. Photonics 4, 780–785 (2010).

    Article  CAS  Google Scholar 

  3. Planchon, T. A. et al. Nat. Methods 8, 417–423 (2011).

    Article  CAS  Google Scholar 

  4. Chen, B.-C. et al. Science 346, 1257998 (2014).

    Article  Google Scholar 

  5. Chang, B.-J. et al. Nat. Methods 16, 235–238 (2019).

    Article  Google Scholar 

  6. Liu, T.-L. et al. Science 360, eaaq1392 (2018).

    Article  Google Scholar 

Download references

Acknowledgements

The author acknowledges J. Madrid-Wolff for comments.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Manu Forero-Shelton.

Ethics declarations

Competing interests

The author declares no competing interests.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Forero-Shelton, M. Peering into cells at high resolution just got easier. Nat Methods 16, 293–294 (2019). https://doi.org/10.1038/s41592-019-0373-3

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/s41592-019-0373-3

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing