Supplementary Figure 5: The FPbase FRET calculator. | Nature Methods

Supplementary Figure 5: The FPbase FRET calculator.

From: FPbase: a community-editable fluorescent protein database

Supplementary Figure 5

The FPbase FRET calculator shows spectral overlap and calculates the Förster distance (R0) for any FP pair in the database (provided the spectra and necessary attributes have been entered). The user may change values for the refractive index of the medium (n) or the orientation factor (κ2). The table below shows precalculated Förster distance measurements for all monomeric FP pairs in the database; clicking on the eye icon in the “Show” column displays that FP pair on the graph.

Back to article page