Supplementary Figure 3: pGT vectors were transferred from E. coli donors to representative recipient species during in vitro conjugations. | Nature Methods

Supplementary Figure 3: pGT vectors were transferred from E. coli donors to representative recipient species during in vitro conjugations.

From: Metagenomic engineering of the mammalian gut microbiome in situ

Supplementary Figure 3

(a) In vitro conjugation efficiency of replicative vector pGT-B1 from E. coli donor to various recipients, which are plotted by phylogenetic relationships. (b) In vitro conjugation efficiency of vector pG-Ah1 between E. coli donor and various recipients. This vector is replicative only in Proteobacteria (E. coli, S. enterica, V. cholerae, P. aeruginosa) but delivered genetic cargo by transposition into a broader array of bacteria. Asterisks indicate cultures grown in anaerobic conditions; all other cultures were grown aerobically. Conjugation efficiencies were calculated from 2 independent conjugations.

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