Fig. 1: Overview of metagenomic alteration of gut microbiome by in situ conjugation (MAGIC). | Nature Methods

Fig. 1: Overview of metagenomic alteration of gut microbiome by in situ conjugation (MAGIC).

From: Metagenomic engineering of the mammalian gut microbiome in situ

Fig. 1

MAGIC implementation to transfer replicative or integrative pGT vectors from an engineered donor strain into amenable recipients in a complex microbiome. Replicative vectors feature a broad-host-range origin of replication (oriR), whereas integrative vectors contain a transposable Himar cassette and transposase (Tnase). The donor E. coli strain contains genomically integrated conjugative transfer genes (tra) and an mCherry gene (mCh). Transconjugant bacteria are detectable on the basis of expression of an engineered payload that includes GFP and an antibiotic-resistance gene (AbR).

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