Fig. 1: Overview of scarless editing. | Nature Methods

Fig. 1: Overview of scarless editing.

From: Efficient scarless genome editing in human pluripotent stem cells

Fig. 1

a, Workflow of the scarless-editing procedure through two-step HR. b, Donor DNA constructs and gRNAs for two-step HR. In the case of TBX1 editing, the intended mutation was G-to-A substitution at 928 bp of the TBX1 cDNA coding site (TBX1 c.928G>A). Arrows indicate the design of the primer for genotyping. WT, wild type. c, Editing timeline. The total estimated time is 1.5–2 months, depending on the growth rate of the cells.

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