Supplementary Figure 5: FLIRT targeting cell division at lower hold temperature and temperature-sensitive mutant specificity. | Nature Methods

Supplementary Figure 5: FLIRT targeting cell division at lower hold temperature and temperature-sensitive mutant specificity.

From: FLIRT: fast local infrared thermogenetics for subcellular control of protein function

Supplementary Figure 5

a, Schematics (left) and representative images from FLIRT experiments targeting to ~25.5 °C in either control (top row) or myosin-II(ts) (bottom three rows) one-cell embryos either on one side of the cell equator or a cell pole with a hold temperature of 14 °C and 10.1 mW of laser power (see Supplementary Fig. 3e), either throughout cell division (see Supplementary Video 4) or for an 8-min window (~6 min after anaphase onset, bottom row; see Supplementary Video 6). b, Schematic (left) and representative images from FLIRT experiments targeting the cell equator in one-cell Delta(ts) embryos. White dashed circles indicate FLIRT-targeted ROIs. Time is in seconds after FLIRT initiation. The number of biologically independent experimental replicates that completed cell division is indicated below each experimental schematic (left). Scale bar, 10 µm.

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