Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Mutation frequency is not increased in CRISPR–Cas9-edited mice

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

Fig. 1: Parent–progeny study.

Data Availability

The data are available at SRA under project number PRJNA470569.

References

  1. 1.

    Schaefer, K. A. et al. Nat. Methods 14, 547–548 (2017).

    CAS  Article  Google Scholar 

  2. 2.

    Anonymous. Nat. Methods 15, 229 (2018).

  3. 3.

    McKenna, A. et al. Genome Res. 20, 1297–1303 (2010).

    CAS  Article  Google Scholar 

  4. 4.

    Van der Auwera, G. A. et al. Curr. Protoc. Bioinformatics 43, 11.10.1–11.10. 33 (2013).

    Google Scholar 

  5. 5.

    DePristo, M. A. et al. Nat. Genet. 43, 491–498 (2011).

    CAS  Article  Google Scholar 

  6. 6.

    Layer, R. M., Chiang, C., Quinlan, A. R. & Hall, I. M. Genome Biol. 15, R84 (2014).

    Article  Google Scholar 

  7. 7.

    Shin, H. Y. et al. Nat. Commun. 8, 15464 (2017).

    CAS  Article  Google Scholar 

  8. 8.

    Iyer, V. et al. PLoS Genet. 14, e1007503 (2018).

    Article  Google Scholar 

  9. 9.

    Luo, X. et al. bioRxiv Preprint at https://www.biorxiv.org/content/early/2018/06/05/339143 (2018).

Download references

Acknowledgements

This study was funded through the IRPs of NIDDK and NHLBI. Research support was received from the NIH Director’s Challenge Innovation Award program (to L.H.). We also thank the team of the NIH HPC Biowulf cluster, especially W. Resch, for their advice, as well as their continued maintenance of hardware and software. We acknowledge the Genomics Platform at The Broad Institute for their support and services provided for the generation of the WGS data.

Author information

Affiliations

Authors

Contributions

M.W., H.E.S., C.L., C.W. and L.H. designed the study. C.L. and C.W. conducted mouse experiments. M.W. and H.E.S. carried out computational analyses. M.W., H.E.S. and L.H. wrote the manuscript.

Corresponding authors

Correspondence to Michaela Willi or Lothar Hennighausen.

Ethics declarations

Competing interests

The authors declare no competing interests.

Integrated supplementary information

Supplementary Figure 1 Equivalence test.

(a) The equivalence test for the de novo SNPs is significant, which verifies that the number of de novo SNPs is equivalent. (CRISPR and control group: n = 6; δ = +/- 1.35) (b) The equivalence test for the de novo indels is inconclusive, as the 90% CI reaches over zero and the lower boundary (CRISPR and control group: n = 6; δ = +/- 0.82).

Supplementary information

Supplementary Text and Figures

Supplementary Figure 1, Supplementary Methods, Supplementary Notes 1–4 and Supplementary Tables 1–7

Reporting Summary

Supplementary Data

De novo mutations in CRISPR–Cas9-edited and control mice

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Willi, M., Smith, H.E., Wang, C. et al. Mutation frequency is not increased in CRISPR–Cas9-edited mice. Nat Methods 15, 756–758 (2018). https://doi.org/10.1038/s41592-018-0148-2

Download citation

Further reading

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing