Supplementary Figure 12: In vitro affinity of AGO2–miR-21 for the ZIKV 5′ CS. | Nature Methods

Supplementary Figure 12: In vitro affinity of AGO2–miR-21 for the ZIKV 5′ CS.

From: COMRADES determines in vivo RNA structures and interactions

Supplementary Figure 12

(a), Electrophoretic mobility shift assay (EMSA) of 5′-labeled miR-21 (lanes 1, 2) upon addition of ZIKV 5′ CS (lane 2); and 5′-labeled ZIKV 5′ CS (lanes 3-5) upon addition of miR-21 (lane 4) or of Ago2-miR-21 complexes (lane 5). The experiment was independently repeated 3 times with similar results. b-h, In vitro measured affinities of Ago2-miR-21 to a wildtype ZIKV 5′ CS. Mean and s.e.m. of 3 independent samples are shown. (b), mutated 5′ CS (c-d), a fully seed matched mutated 5′ CS (e), mutated 5′ CS with no cHP stem-loop (f), extended 5′ CS sequence containing the DCS stem-loop (g), and a short target perfectly complementary to the miR-21 seed (h).