Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

Chemical Biology

Controlling protein function with HCV protease

Two methods for controlling protein activities with small molecules provide a general solution to a long-standing challenge in mammalian synthetic biology.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Fig. 1: In LInC and StaPLs, a linker using HCV protease and its cognate recognition sequence is installed between a protein of interest and a regulatory domain.

Marina Corral Spence/Springer Nature

References

  1. Tague, E. P., Dotson, H. L., Tunney, S. N., Sloas, D. C. & Ngo, J. T. Nat. Methods https://doi.org/10.1038/s41592-018-0042-y (2018).

  2. Jacobs, C. L., Badiee, R. K. & Lin, M. Z. Nat. Methods https://doi.org/10.1038/s41592-018-0041-z (2018).

  3. Ruder, W. C., Lu, T. & Collins, J. J. Science 333, 1248–1252 (2011).

    Article  PubMed  CAS  Google Scholar 

  4. Lienert, F., Lohmueller, J. J., Garg, A. & Silver, P. A. Nat. Rev. Mol. Cell Biol. 15, 95–107 (2014).

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  5. Spencer, D. M., Wandless, T. J., Schreiber, S. L. & Crabtree, G. R. Science 262, 1019–1024 (1993).

    Article  PubMed  CAS  Google Scholar 

  6. Rakhit, R., Navarro, R. & Wandless, T. J. Chem. Biol. 21, 1238–1252 (2014).

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  7. McCauley, J. A. & Rudd, M. T. Curr. Opin. Pharmacol. 30, 84–92 (2016).

    Article  PubMed  CAS  Google Scholar 

  8. Lin, M. Z., Glenn, J. S. & Tsien, R. Y. Proc. Natl Acad. Sci. USA 105, 7744–7749 (2008).

    Article  PubMed  Google Scholar 

  9. Chung, H. K. et al. Nat. Chem. Biol. 11, 713–720 (2015).

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  10. Pu, J., Chronis, I., Ahn, D. & Dickinson, B. C. J. Am. Chem. Soc. 137, 15996–15999 (2015).

    Article  PubMed  PubMed Central  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Bryan C. Dickinson.

Ethics declarations

Competing interests

The author declares no competing interests.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Dickinson, B.C. Controlling protein function with HCV protease. Nat Methods 15, 489–490 (2018). https://doi.org/10.1038/s41592-018-0043-x

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/s41592-018-0043-x

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing