We review the use of luminescent nanoparticles in super-resolution imaging and single-molecule tracking, and showcase novel approaches to super-resolution imaging that leverage the brightness, stability, and unique optical-switching properties of these nanoparticles. We also discuss the challenges associated with their use in biological systems, including intracellular delivery and molecular targeting. In doing so, we hope to provide practical guidance for biologists and continue to bridge the fields of super-resolution imaging and nanoparticle engineering to support their mutual advancement.
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The authors acknowledge S. Qu (Changchun Institute of Optics Fine Mechanics and Physics, Chinese Academy of Sciences), C. Wu (Southern University of Science and Technology), Q. Su, O. Shimoni, and F. Wang for valuable technical discussions. D.J., A.M.v.O., and P.X. acknowledge support from the Australian Research Council (FT 130100517, FL140100027) and the National Natural Science Foundation of China (61729501, 51720105015). J.E. is grateful for financial support from the Cluster of Excellence and DFG Research Centre Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB).
The authors declare no competing interests.
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Jin, D., Xi, P., Wang, B. et al. Nanoparticles for super-resolution microscopy and single-molecule tracking. Nat Methods 15, 415–423 (2018). https://doi.org/10.1038/s41592-018-0012-4
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