Impact of a TLR9 agonist and broadly neutralizing antibodies on HIV-1 persistence: the randomized phase 2a TITAN trial

Inducing antiretroviral therapy (ART)-free virological control is a critical step toward a human immunodeficiency virus type 1 (HIV-1) cure. In this phase 2a, placebo-controlled, double-blinded trial, 43 people (85% males) with HIV-1 on ART were randomized to (1) placebo/placebo, (2) lefitolimod (TLR9 agonist)/placebo, (3) placebo/broadly neutralizing anti-HIV-1 antibodies (bNAbs) or (4) lefitolimod/bNAb. ART interruption (ATI) started at week 3. Lefitolimod was administered once weekly for the first 8 weeks, and bNAbs were administered twice, 1 d before and 3 weeks after ATI. The primary endpoint was time to loss of virologic control after ATI. The median delay in time to loss of virologic control compared to the placebo/placebo group was 0.5 weeks (P = 0.49), 12.5 weeks (P = 0.003) and 9.5 weeks (P = 0.004) in the lefitolimod/placebo, placebo/bNAb and lefitolimod/bNAb groups, respectively. Among secondary endpoints, viral doubling time was slower for bNAb groups compared to non-bNAb groups, and the interventions were overall safe. We observed no added benefit of lefitolimod. Despite subtherapeutic plasma bNAb levels, 36% (4/11) in the placebo/bNAb group compared to 0% (0/10) in the placebo/placebo group maintained virologic control after the 25-week ATI. Although immunotherapy with lefitolimod did not lead to ART-free HIV-1 control, bNAbs may be important components in future HIV-1 curative strategies. ClinicalTrials.gov identifier: NCT03837756.

Human immunodeficiency virus type 1 (HIV-1) infection persists mainly in CD4 + T cells as a long-lived HIV-1 reservoir. Continuous lifelong antiretroviral therapy (ART) is needed to suppress viral replication and prevent disease progression. When ART is stopped, viral replication quickly resumes, leading to rebound of plasma viremia within weeks in almost all individuals [1][2][3][4][5] .
Many HIV-1 curative strategies aim to enhance HIV-1-specific immunity as a means to achieve ART-free HIV-1 virologic control. Until recently, HIV-1 cure trials have typically involved people with HIV-1 (PWH) on long-term suppressive ART, which is continued during the therapeutic interventions 6 . In these individuals, the level of HIV-1 antigen both in blood and tissues is extremely low due to the effective modern ART regimens. The scarcity of antigen may explain why experimental immunotherapies, such as cytokines and other immunostimulatory agents, have had limited impact on HIV-1-specific immunity and viral persistence in clinical trials 7,8 .

Plasma HIV-1 RNA kinetics after ART interruption
To determine the impact of the interventions on ART-free virologic control, all participants underwent a closely monitored 25-week ATI. The study's primary endpoint was time from stopping ART to the date of meeting the criteria for loss of virologic control (defined as 4 weeks with sustained plasma HIV-1 RNA ≥1,000 copies per milliliter or two consecutive measurements >100,000 copies per milliliter). All participants in the placebo/placebo group experienced loss of virologic control, which occurred at a median of 4.5 weeks (IQR: 3.0-11) after stopping ART (Fig. 2a). In the lefitolimod/placebo group, median time to loss of virologic control was 5.0 weeks (IQR: 4.0-6.0) (Fig. 2b). One individual in this group had partial ART-free virologic control with viremia ranging from <20 to 5,500 copies per milliliter throughout the ATI (Fig. 2b). In the placebo/bNAb group, median time to loss of virologic control was 17 weeks (IQR: [11][12][13][14][15][16][17][18][19][20][21][22][23][24][25] (Fig. 2c). However, four of 11 participants (36%) maintained virologic control during the 25-week ATI. In the lefitolimod/bNAb group, the median time to reach criteria for viral rebound was 14 weeks (IQR: 10-17) (Fig. 2d). One individual had partial ART-free virologic control, with viremia ranging from <20 to 65,100 copies per milliliter during the ATI. All six individuals with either partial or complete ART-free virologic control tested negative for antiretroviral drugs in plasma at week 25 of the ATI. One partial controller in the placebo/bNAb group had a protective HLA class I allele (ID601: HLA*B27) (Extended Data Table 1), and one started ART less than 6 months from the presumed date of HIV-1 infection (ID142) (Extended Data Table 1).
Although there was no difference in time to loss of virologic control between the lefitolimod/placebo group and the placebo/placebo group (P = 0.49) (Fig. 2e), loss of virologic control occurred significantly later in both the placebo/bNAb group (median delay, 12.5 weeks, P = 0.003) and the lefitolimod/bNAb group (median delay, 9.5 weeks, P = 0.004) compared to the placebo/placebo group (Fig. 2f,g). There was no difference in time to loss of virologic control between the placebo/bNAb and lefitolimod/bNAb group (P = 0.42) (Fig. 2h). Similar differences were observed when we analyzed time to first plasma HIV-1 RNA measurement >50 or >1,000 copies per milliliter (Extended Data Fig. 2). Of note, the median initial viral doubling time, defined as the trajectory of the increase of the initial slope in plasma HIV-1 RNA during ATI, was slower for the two bNAb groups compared to non-bNAb groups (Fig. 2i). Median initial viral doubling time was significantly slower for the lefitolimod/bNAb group compared to the placebo/ placebo (P = 0.030) and lefitolimod/placebo (P = 0.0026) groups. The median initial viral doubling time strongly correlated with time to loss of virological control (P < 0.0001) (Fig. 2j).
In the placebo/bNAb group, two of the four individuals who did not meet criteria for loss of virologic control displayed partial virologic control with viremia ranging from <20 to 70,000 copies per milliliter, whereas the other two individuals maintained complete virologic control with undetectable plasma HIV-1 RNA levels. These two individuals with undetectable viral load elected not to restart ART after the end of the ATI and continue to control under close monitoring 18 months after stopping ART (Extended Data Fig. 3). Collectively, these findings demonstrate that, in a placebo-controlled setting, administration of two bNAbs significantly delayed viral rebound and was associated with partial or complete ART-free virologic control among a subset of individuals. Lefitolimod did not impact the outcome of the ATI.
suppressive ART induced activation of pDCs, leading to potent upregulation of antiviral immune responses [12][13][14][15] . During TLR9 agonist treatment, the proportion of activated cytotoxic (CD56 dim CD16 + ) natural killer (NK) and CD8 + T cells expanded in blood 12,13 and lymph nodes 14 . This might facilitate killing of infected cells, as ex vivo studies have shown that priming of effector cells, such as cytotoxic T lymphocytes (CTLs) and NK cells, enhances their ability to recognize and eliminate antigen-expressing infected cells [16][17][18][19][20] . Finally, TLR9 agonist treatment may directly impact the HIV-1 reservoir through cytokine release or cell-to-cell-mediated activation 21 . In a small clinical trial, an increased frequency in viral blips during lefitolimod administration was observed among PWH on ART, suggesting that lefitolimod may have a latency-reversing effect on the HIV-1 reservoir 12 .
The immunological effects of lefitolimod could potentially be boosted either by administering the drug in the presence of higher antigen load or by co-administration with broadly neutralizing anti-HIV-1 antibodies (bNAbs), such as 3BNC117 and 10-1074, which recognize non-overlapping epitopes on the HIV-1 envelope protein gp120 (refs. 22,23). In support of the latter concept, non-human primate (NHP) studies have shown that administration of a TLR7 agonist, which, similarly to a TLR9 agonist, activates pDCs and triggers antiviral immune responses, in combination with bNAbs led to ART-free virologic control in a large proportion of animals during ART interruption (ATI) [24][25][26] . In phase 1-2 clinical trials, repeated administration (3-8 doses) of the combination of 3BNC117 and 10-1074 maintained viral suppression for ~13 weeks after last bNAb infusion when administered instead of ART [27][28][29] . Finally, owing to Fc-mediated engagement of effector and antigen-presenting cells, bNAbs independently exert immunomodulatory effects, and bNAb treatment has been shown to augment HIV-1-specific T cell immunity both in viremic NHPs and in humans 6,[30][31][32] .
Collectively, these observations led us to hypothesize that lefitolimod alone or in combination with two doses of 3BNC117 and 10-1074 administered in the setting of ATI could potentially enhance elimination of infected cells and boost HIV-1-specific CD8 + T cell immunity, leading to ART-free virologic control (Fig. 1a) 33 . To address this hypothesis, we conducted an investigator-initiated, randomized, placebo-controlled, double-blinded phase 2a trial to determine the impact of lefitolimod and 3BNC117 + 10-1074 on virologic control among PWH undergoing ATI. Secondary endpoints were safety and viral rebound kinetics.

Participants and follow-up
Eligible individuals were recruited from 12 April 2019 to 5 November 2021, and follow-up concluded on 9 June 2022. Of 117 individuals who were screened for sensitivity to 3BNC117 and 10-1074 by phenotypic or sequence-based analysis, 46 participants were enrolled in the study, of whom three withdrew consent for personal reasons and did not receive the random allocation treatment ( Fig. 1b and Extended Data Fig. 1). Of the remaining 43 participants, 10 were randomly allocated to placebo/ placebo, 10 to lefitolimod/placebo, 11 to placebo/bNAb and 12 to lefitolimod/bNAb. Lefitolimod (120 mg) or placebo was dosed subcutaneously (s.c.) once weekly for 8 weeks starting 2 weeks before the ATI and ending at the beginning of week 6 of the ATI (Fig. 1a). 3BNC117 (30 mg kg −1 ) and 10-1074 (20 mg kg −1 ) or placebo were given as sequential intravenous (i.v.) infusions the day before starting the ATI and 3 weeks into the ATI. One participant in the placebo/bNAb group discontinued the ATI and re-started ART after an infusion-related reaction during the second bNAb infusion (Fig. 1b). This person was included in the analyses. No participants were lost to follow-up.

bNAb sensitivity and serum concentrations
The proviral reservoir's sensitivity to neutralization by 3BNC117 and 10-1074 was assessed for each potential study participant as part of the inclusion criteria before randomization (see Methods for details). bNAb sensitivity screening was primarily done using the PhenoSense Monoclonal Antibody Assay (LabCorp-Monogram Biosciences). If phenotypic sensitivity results were inconclusive or the PhenoSense assay could not be carried out, single-genome amplification and sequencing of HIV-1 envelope (env) followed by genotypic sensitivity prediction was used as an alternate screening method ( Fig. 3 and Extended Data Fig. 4). Among participants who received bNAb, plasma HIV env sequences were analyzed for known resistance mutations if viral rebound occurred (Fig. 3). As serum levels of 3BNC117 gradually declined to subtherapeutic levels, most participants in the bNAb groups experienced viral rebound while seemingly on 10-1074 monotherapy (Fig. 4a,b). Post hoc analyses demonstrated that the prevalence of genotypic 10-1074 resistance mutations increased from 13% at screening to 89% at viral rebound among bNAb-treated individuals (Fig. 3). At the time of loss of virologic control, the mean serum concentrations were 13.2 µg ml −1 (s.d. ± 10.6) for 3BNC117 and 73.3 µg ml −1 (s.d. ± 47.2) for 10-1074 in the placebo/bNAb group and 20.1 µg ml −1 (s.d. ± 21.0) and 88.2 µg ml −1 (s.d. ± 70.1), respectively, for the lefitolimod/bNAb group. Of note, in a mixed-effects model, the decline in serum bNAb concentrations after the second antibody dose was significantly faster in the lefitolimod/bNAb group compared to the placebo/bNAb group (P = 0.023 for 3BNC117 and P = 0.019 for 10-1074) (Fig. 4c,d).  Allocated to placebo/bNAb (n = 12)

Fig. 1 | TITAN trial design (a) and abbreviated CONSORT flow diagram (b).
Solid green arrows indicate lefitolimod injections. Solid red and blue triangles indicate 3BNC117 and 10-1074 infusions, respectively. Empty green arrows as well as red and blue triangles indicate placebo injections or infusions, respectively.
Gray shaded areas indicate time on ART, and white shaded areas indicate still interrupting ART during the 25 weeks of ATI. The analysis section is presented in full in Extended Data Fig. 1. SAE, severe adverse event.
Article https://doi.org/10.1038/s41591-023-02547-6 Although their reservoir was predicted to be sensitive based on phenotypic screening, the three bNAb recipients (ID133, ID101 and ID822) with the shortest time to loss of virologic control (<8 weeks) were subsequently shown to harbor genotypic 10-1074-resistant proviruses at enrollment (Fig. 3). Notably, the two individuals with complete virologic control were enrolled based on their genotypic bNAb sensitivity screening, but post hoc phenotypic data indicated some level of resistance to either 3BNC117 or 10-1074 at enrollment. In summary, despite careful screening for bNAb sensitivity before enrollment, some participants appeared to harbor resistant proviruses that resulted in early viral rebound. These observations highlight the challenges with the current approaches to DNA-based bNAb sensitivity screening in PWH on long-term ART.

Quantification of intact HIV proviruses
We used a digital droplet PCR (ddPCR) assay (Intact Proviral DNA Assay (IPDA)) to estimate the proportion of intact proviruses in CD4 + T cells. For some (9/31) individuals with subtype B for whom IPDA failed, as well as for the individuals with non-B subtypes (n = 6), we used alternative primer/probe or an IPDA-like duplexed ddPCR (3dPCR) assay (Supplementary Table 1) to estimate the intact reservoir 6 . The number of intact proviruses varied among individuals at baseline (range: 1.18-2,576.62 copies per 10 6 CD4 + T cells; Extended Data Table 1) but did not differ between groups (P = 0.50). The mean proportion of intact proviruses at baseline was 7% (Extended Data Fig. 5a). Six weeks after starting ATI, the placebo/placebo and lefitolimod/placebo groups showed significant increases in circulating intact and defective proviruses ( Fig. 5a and Extended Data Fig. 5b,c). At this time, 16 of 20 individuals in the placebo/ placebo and lefitolimod/placebo groups had reached criteria for loss of virologic control (Fig. 2a,b). The largest median increase in intact and defective proviruses was observed among the lefitolimod/placebo group ( Fig. 5b and Extended Data Fig. 5d,e). The median increase in intact proviruses among the placebo/placebo group was significantly higher than the median changes in the two bNAb groups (Fig. 5b). Similar changes were observed for defective proviruses (Extended Data Fig. 5d,e). In contrast, we found no significant increases in intact or defective proviruses in the bNAb recipients over the same time period, suggesting that the two bNAbs restricted HIV-1 replication during ATI. In summary, we observed that the frequency of CD4 + T cells harboring intact proviruses expanded after ATI in non-bNAb recipients but that bNAb administration prevented reservoir expansion during the early phase of the ATI.

HIV-1-specific T cell immunity
The median frequency of HIV-1-specific CD8 + T cells increased from pre-ATI to week 6 in all four treatment groups as measured by the activation-induced marker (AIM) assay, but the increase was significant only for the lefitolimod/placebo group (Fig. 5c). This change in HIV-1-specific CD8 + T cell responses from pre-ATI to week 6 was significantly correlated with measurable plasma HIV-1 RNA at week 6 (r = 0.51, P = 0.021) (Extended Data Fig. 6a). The frequency of HIV-1-specific CD8 + T cells also increased significantly from the pre-ATI timepoint to week 13 for the lefitolimod/bNAb group (P = 0.0059). To disentangle increased antigen exposure from a bNAb-mediated vaccinal effect as potential cause of increased HIV-1 specific immunity, we separated bNAb-treated individuals in a post hoc analysis into two groups based on whether their plasma HIV-1 RNA was below or above 50 copies per milliliter during the ATI. As expected, bNAb-treated individuals with plasma HIV-1 RNA above 50 copies per milliliter had higher HIV-1-specific CD8 + T cell responses at week 13 compared to pre-ATI (P = 0.0052) (Fig. 5d). We also observed a non-significant increase in total HIV-1-specific CD8 + T cell responses among bNAb-treated individuals whose plasma HIV-1 RNA was below 50 copies per milliliter at week 13 (P = 0.094) (Fig. 5d), but we found no detectable changes in Gag-specific CD8 + T cell responses or in Gag-induced interferon (IFN)-γ or granzyme B (GzmB) responses (Extended Data Fig. 6b-d). The frequency of HIV-1-specific CD4 + T cells remained relatively stable over time in all four groups (Extended Data Fig. 6e). Thus, whereas HIV-1-specific CD8 + T cell responses increased with increasing plasma HIV-1 RNA during the ATI, bNAb recipients who maintained plasma HIV-1 RNA <50 copies per milliliter during 3 months of ATI did not experience substantial increases in their HIV-1-specific CD8 + T cell responses that would be suggestive of a bNAb-mediated  to loss of virologic control for the three active interventional groups compared to the placebo/placebo group: lefitolimod/placebo (e), placebo/bNAb (f) and lefitolimod/bNAb (g). h, Time to loss of virologic control for the placebo/bNAb group compared to the letifolimod/bNAb group. P values were calculated using the log-rank test. i, Dot plot of the initial viral doubling time in plasma HIV-1 RNA during ATI among the four randomization groups. The number of individuals per group is as stated for a-d, but the two individuals with completed virologic control in the placebo/bNAb group were given a high doubling time of 100 d beyond the axis to be included and to avoid skewing the data (lines at median and IQRs). P values comparing between groups were calculated using the two-tailed Mann-Whitney test. j, Correlation between time to loss of ART-free virologic control during the 25 weeks of ATI and initial viral doubling time among the four randomization groups. The number of individuals per group is as stated in i. P value was calculated using two-tailed Spearman's correlation coefficient.

Safety
Both lefitolimod and bNAbs were overall safe. A total of 253 adverse events (AEs) were registered, of which 94 were determined to be unrelated to any investigational drug, including placebo (Extended Data  Tables 2 and 3). Of 81 AEs considered related to lefitolimod, 75 were graded mild and six were graded moderate, with the most common AEs being injection site reaction (n = 39) and fatigue (n = 6). Of 14 AEs considered related to bNAb, 11 were graded mild, two were graded moderate and one was graded severe, with fatigue (n = 5) being the most commonly reported AE. An infusion-related reaction categorized as severe and related to the second 3BNC117 infusion (ID133) resolved with fluid therapy, antihistamine and corticosteroid without any sequelae. Another AE, not related to study drugs, was categorized  ID101   ID822   ID815   ID109   ID119   ID801   ID143   ID609   ID809   ID807   ID412   ID204   ID805   ID139   ID312   ID813   ID117   ID106   ID601   ID114   ID314   ID142   ID133   30    bNAb sensitivity was primarily analyzed using the PhenoSense Monoclonal Antibody Assay with predefined IC 90 thresholds for 3BNC117 (<1.5 µg ml −1 ) and 10-1074 (<2.0 µg ml −1 ) and MPI ≥98%. In six participants, the PhenoSense Assay failed, and we secondarily used proviral HIV-1 env sequences on a genotypic prediction algorithm with predefined thresholds of >90% known sequences are sensitive. One participant (ID142) was enrolled based upon the genotypically analysis, but post hoc phenotypic data showed an IC 90 of 1.8 µg ml −1 for 3BNC117. In another participant (ID314), both the PhenoSense Assay and env sequencing initially failed, and we tertiary (Methods) included this individual based on the assumption that both assays failed due to a very small reservoir size, but post hoc phenotypic data showed archived 10-1074-resistant proviruses. For individuals enrolled based upon the phenotypic data, genotypical data were subsequently obtained except for one participant (ID314). If the participants had reached criteria for viral rebound before or were viremic at the end of the 25 weeks of ATI, bNAb sensitivity using plasma HIV-1 env sequences was analyzed using the same genotypic prediction algorithm. as severe, where a participant (ID813) had a vasovagal reaction just after insertion of the intravenous needle before bNAb infusion. The reaction resolved with fluid therapy without any sequelae. Median CD4 + T cell counts decreased from study start to end of study in three of the four groups (Extended Data Fig. 7) but was stable in the placebo/bNAb group. Among participants who re-initiated ART after viral rebound, all but one (ID807) achieved viral re-suppression after a median of 71 d (IQR: , and median CD4 + T cell counts had recovered to pre-ATI levels in all four groups (Extended Data Fig. 7b).

Discussion
In this randomized, placebo-controlled HIV-1 cure trial among PWH on long-term ART who screened sensitive to the study bNAbs, we found that two doses of 3BNC117 and 10-1074 in the setting of ATI led to a significant delay in viral rebound compared to placebo. We also observed that 36% of individuals in the placebo/bNAb group compared to none in the placebo group maintained partial or complete virologic control at the end of the 25-week ATI. There were no differences in time to viral rebound or change in reservoir size between the placebo/placebo and the lefitolimod/placebo group or between the placebo/bNAb and lefitolimod/bNAb group. Thus, although there was no added clinical or immunological benefit of combining lefitolimod with bNAbs, antibody treatment alone led to a significant delay in viral rebound, and some bNAb-treated individuals experienced long-term ART-free virologic control.
Single-arm trials show that bNAb-sensitive PWH on long-term ART receiving 3-8 infusions of 3BNC117 and 10-1074 maintain prolonged viral suppression in the absence of ART [27][28][29] . Notably, our study was not designed to evaluate the ability of bNAbs to replace ART as a means to durably suppress viral replication but, rather, to address an important HIV-1 cure hypothesis: could a TLR9 agonist alone or in combination with bNAbs increase the chance of long-term ART-free control? We addressed this question using the most rigorous study design: a factorial, randomized, placebo-controlled, double-blinded trial. We administered bNAbs only twice-one infusion before stopping ART and a second infusion 3 weeks after ATI-but found that time to viral rebound after the last infusion was similar to that seen in previous multi-dose trials [27][28][29] .
Of note, we administered 10-1074 at 20 mg kg −1 compared to 30 mg kg −1 for 3BNC117 to compensate for the longer half-life of 10-1074 compared to 3BNC117, but, despite differential dosing, 3BNC117 was cleared from plasma faster than 10-1074, leading to a period of effective monotherapy with the latter. Consistent with the pharmacokinetics, 89% of our participants developed new 10-1074 resistance mutations at viral rebound indicative of residual 10-1074 monotherapy, which has also been reported in other trials. Collectively, these studies demonstrate that 10-1074 monotherapy is not sufficient to maintain virologic suppression 34 .
In our study, 18% of participants (2/11) in the placebo/bNAb group maintained complete virologic control (HIV RNA <20 copies per milliliter) throughout the 25-week ATI, and both elected to continue off ART after the ATI ended. In previous trials using the same bNAb combination, 0% (0/7) 28 , 11% (1/9) 27 and 17% (2/12) 29 of bNAb-sensitive individuals maintained complete virologic control during ATI. Furthermore, in our study, bNAb treatment changed the trajectory of viral rebound as evidenced by slower plasma virus doubling time during the ATI, which is suggestive of stronger immune pressure against the replicating virus in bNAb recipients.
Although virologic or immunological features characteristic of post-bNAb controllers have yet to be identified, pre-ATI HIV-1 reservoir size was seven-fold lower in post-ART controllers compared to non-controllers in a cohort study 35 . Further analysis showed that the reservoir in post-treatment controllers remained stable during ATI 36 . We found that the frequency of intact HIV-1 in CD4 + T cells remained stable during bNAb-mediated suppression of plasma viral load but expanded upon loss of virologic control. The two individuals who maintained complete virologic control maintained a stable reservoir throughout the ATI.
As expected, we found that HIV-1-specific CD8 + T cell responses increased during viral rebound. We also observed a modest increase in HIV-1-specific CD8 + T cell responses among bNAb recipients who maintained viral suppression, but, in contrast to a previous report 37 , we observed no change in Gag-specific CD8 + T cell responses or Gag-induced cytokine release. Thus, there was no clear indication of a vaccinal effect 31,[37][38][39][40][41][42] in the present study. The failure to broadly stimulate cellular immunity could be due to low amounts of antigen at the time of bNAb administration because bNAb dosed at ART initiation among newly diagnosed viremic individuals induced sustained strong HIV-1-specific CD8 + T cell responses 6,30 . Potent HIV-1-specific CD8 + T cell immunity is critical for maintaining virologic suppression in humans and bNAb-treated macaques 31,32,43 . Whether increases in HIV-1-specific cellular immunity after bNAb administration contributed to virologic control in the present study is unclear, but it seems plausible that a combined restriction of outgrowth from the viral reservoir, along with potent cellular responses, contributed to long-term ART-free control in some individuals.
The combination of 3BNC117 and 10-1074 neutralizes a broad range of HIV-1 subtypes ex vivo 44 , but bNAb treatment is still challenged by archived resistant mutations 29,45 . To address this issue, we pre-screened all participants for bNAb sensitivity using both genotypic and phenotypic approaches. However, neither bNAb sensitivity analysis accurately predicted time to viral rebound, adding further evidence of the challenge of effective screening for bNAb sensitivity. This finding is in line with other recent studies, which indicate that currently available bNAb sensitivity testing of the proviral reservoir does not accurately predict the clinical outcome of bNAb treatment 6,29 . Of note, the average serum concentrations of 3BNC117 at viral rebound were slightly higher than previously reported, where viral rebound generally occurred after 3BNC117 decreased below 10 µg ml −1 (refs. 27,29). However, both studies were relatively small, and serum antibody concentrations were measured by different methods.
Lefitolimod alone or in combination with bNAbs did not significantly impact time to viral rebound or any of the immunological or virologic outcomes in this trial. Unexpectedly, co-administration of lefitolimod led to a faster decline in bNAb serum concentrations 24,25 . Lefitolimod administration has been shown to increase proportions of CD16 + NK cells among PWH [12][13][14] , which theoretically could facilitate increased binding and faster clearance of bNAbs. Of note, a faster  decline in serum concentration of an Fc-modified version of PGT121, a bNAb with similar properties to 10-1074, was also observed when it was co-administered with a TLR7 agonist in NHPs 26 . Anti-drug antibodies (ADAs) against anti-HIV-1 bNAbs rarely develop in humans, and, when they do, the presence of ADA has discernible impact on bNAb elimination kinetics 44 .
The combination of a TLR7 agonist and PGT121 was shown to induce long-term ART-free virologic control among 34-45% of NHPs 24,46 , so why did a TLR9 agonist in combination with two bNAbs fail to produce similar effects in humans? Potential explanations include the lower degree of fitness and greater bNAb sensitivity of simianhuman immunodeficiency virus (SHIV) versus HIV-1, a smaller reservoir in macaques and very early ART initiation (day 7 or day 9 after infection) in the NHP model. In contrast, our study population consisted mainly of people who started ART during the chronic phase of infection and had large genetically diverse reservoirs with varying degrees of bNAb sensitivity. Early ART initiation and bNAb sensitivity are two key parameters that determine the outcome of ATIs in bNAb studies in humans and NHPs. In two complementary studies with NHPs that started ART later (day 14 and day 365 after infection), the combination of TLR7 agonist and one or more bNAbs tended to be associated with a lower frequency of post-intervention control (0%, 22% and 50%) than in the studies where animals started ART at day 7 or day 9 after infection 25,26 .
Another potential explanation is differences in immune signaling between TLR7, which recognizes viral single-stranded RNA, and TLR9, which recognizes unmethylated DNA with CpG motifs 10 . Although both TLR7 and TLR9 are expressed in the endosomal compartment and signal via myeloid differentiation factor 88 (MyD88), there are notable differences 10 . In human peripheral blood mononuclear cells (PBMCs), TLR7 agonists induce a rapid burst of type I IFN transcripts, whereas TLR9 agonists trigger a slower but sustained expression of type I IFN 47 . The subtypes of induced type I IFNs also differ between the two agonists. Collectively, these experimental and biological differences may explain the divergent results between macaque and human HIV-1 cure trials.
Despite lefitolimod's well-documented ability to enhance DC-mediated cross-presentation, upregulate IFN-stimulated genes and activate multiple innate and adaptive immune cell subsets 12-15 , we   ; lefitolimod/ placebo (n = 9); placebo/bNAb (n = 10); and lefitolimod/bNAb (n = 11). All weeks are shown, including weeks with no data available. P values comparing within groups were calculated using the paired two-tailed Wilcoxon test. Only P values below 0.05 are shown. b, Median change in the intact HIV-1 reservoir between week 6 of the ATI and pre-ATI for the four randomization groups are shown. Data are median and IQRs with the placebo/placebo group as the reference. Placebo/placebo group (n = 10); lefitolimod/placebo (n = 8); placebo/bNAb (n = 9); and lefitolimod/bNAb (n = 9). P values comparing between groups were calculated using the two-tailed Mann-Whitney test. c, Dot plot of the frequency of HIV-1-specific CD8 + T cells at ATI start (day 0) and after 6, 13 and 25 weeks of ATI among individuals in the four randomization groups (lines at median and IQRs): placebo/placebo (n = 10); lefitolimod/placebo (n = 9); placebo/bNAb (n = 11); and lefitolimod/bNAb (n = 12). All weeks are shown, including weeks with no data available. P values comparing within groups and between groups were calculated using the paired two-tailed Wilcoxon test and two-tailed Mann-Whitney test, respectively. Only P values below 0.05 are shown. d, Dot plot of median fold change in HIV-1-specific CD8 + T cells after 13 weeks of ATI among individuals with either plasma HIV-1 RNA below (left) or above (right) 50 copies per milliliter in the two randomization groups receiving bNAb combination (lines at median and IQRs). Plasma HIV-1 RNA below 50 copies per milliliter: placebo/bNAb (n = 5) and lefitolimod/bNAb (n = 1); plasma HIV-1 RNA above 50 copies per milliliter: placebo/bNAb (n = 5) and lefitolimod/bNAb (n = 9). P values comparing within groups were calculated using the paired two-tailed Wilcoxon test.
Article https://doi.org/10.1038/s41591-023-02547-6 observed no discernible impact of TLR9 agonist treatment on T cell immunity. Our findings may indicate that such immunostimulatory molecules may not effectively boost HIV-1-specific immunity unless substantial amounts of HIV-1 antigen are concurrently present in the same anatomical location. This hypothesis gains support from recent discoveries in cancer research, wherein intra-tumoral injection of cytokines or TLR9 agonists has demonstrated substantially greater effects on tumor size and tumor-specific immune responses compared to the same therapies administered s.c. or i.v. 48,49 . However, ongoing trials will yield additional insights into the potential of an HIV-1 cure strategy that centers on the combination of bNAbs with immunostimulatory agents, such as other TLR agonists, pegylated interferon-a2b or IL-15 super-agonists. These trials will determine whether this approach can induce post-treatment control or if it necessitates a re-evaluation of our current strategy. The reported results have limitations and may not be generalizable to all PWH. Specifically, our ability to predict proviral bNAb sensitivity based on the PhenoSense assay or sequence analysis is limited. Undetected bNAb resistance leading to treatment failure may have clinical implications, particularly if bNAbs were to replace modern ART regimens as suppressive antiviral treatment or if used in combination with the new long-acting small-molecule-based injectable antivirals. Most enrolled participants were male. In contrast to some bNAb trials 28 , we did not exclude individuals who initiated ART during the chronic phase of infection. Although this inclusive approach made our study population more representative of the overall population, it might also have contributed to raising the bar for success, as PWH who started ART during the acute/early phase of infection harbor smaller and less diverse HIV-1 reservoirs and may be more likely to become post-treatment controllers [50][51][52] .
Although lefitolimod and bNAbs were overall safe, the CD4 + T cell count dropped in response to recrudescent viremia. Reassuringly, once plasma HIV-1 RNA was re-suppressed, the CD4 + T cell counts returned to pre-ATI levels. We also found that the size of the HIV-1 reservoir expanded at rebound. In a previous study 53 , 22 PWH interrupted ART for a median of 4 months and were followed for ~2 years after restarting ART. The investigators found no difference in HIV-1 reservoir size from pre-ATI and until the last follow-up timepoint. Thus, although viral rebound might cause transient CD4 + cell count declines and HIV-1 reservoir size increases, the long-term clinical implications of these changes, if any, appear to be limited. Of note, one individual in this study (ID807) did not achieve rapid viral re-suppression despite having therapeutic ART drug concentrations and no evidence of emerging or archived resistance mutations. We speculate that this case of slow viral resuppression could be due to clonally expanded defective proviruses, which were recently reported to be a potential source of non-suppressible viremia among fully ART-adherent individuals 54 .
In conclusion, although there was no added benefit of combining a TLR9 agonist with bNAbs compared to bNAbs alone, this was, to our knowledge, the first placebo-controlled, double-blinded trial to show prolonged ART-free virologic control during ATI in individuals receiving just two doses of bNAbs. This finding provides further support for investigating bNAbs as a component in long-acting treatment and HIV-1 curative strategies. However, additional interventions, optimization of bNAbs and bNAb drug combinations 55,56 and/or administration of bNAbs in the setting of higher antigen load will likely be needed to achieve ART-free HIV-1 remission in the majority of PWH on long-term suppressive ART.

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Study design
This was a phase 2a, investigator-initiated, randomized, placebo-controlled, double-blinded international multicenter trial enrolling at six sites in Denmark (Aalborg, Aarhus, Gødstrup, Hvidovre, Odense and Rigshospitalet); one site in Oslo, Norway; and one site in Melbourne, Australia (EudraCT: 2018-001165-16). The first participants were enrolled on 16 May 2019. Before any study-related procedures, written informed consent was obtained from the participants. Participants were randomized into one of four groups-placebo/placebo, lefitolimod/ placebo, placebo/bNAb or lefitolimod/bNAb-in a 1:1:1:1 ratio (Fig. 1a,b). Sex of participants was determined based on self-reporting. The planned sample size was 48 participants. Study procedures were unfortunately severely impacted by the coronavris disease 2019 (COVID-19) pandemic, and enrollment had to be paused for longer periods in 2020 and 2021. Due to expiration dates of the study drugs, enrollment closed on 5 November 2021 with 46 of the 48 planned participants enrolled. Screening took place 2-20 weeks before entering the trial. Study participants remained on ART for the first 2 weeks (week −2 to week 0) of the 8-week interventional period. The 25-week ATI started at week 0; thus, administration of study medication ended in week 5 of the ATI. Regular follow-up visits continued for 20 weeks (weeks 6-25) after the last dose of the study medication. Participants who resumed ART during ATI were followed at 4-week intervals until plasma HIV-1 RNA was <50 copies per milliliter. The study was conducted in accordance with Good Clinical Practice and is reported in accordance with the CONSORT 2010 statement 57

Participants
PWH were aged 18-65 years and on ART for at least 18 months, with plasma HIV-1 RNA <50 copies per milliliter for at least 15 months and a CD4 T cell count >500 cells per mm 3 . Detailed inclusion/exclusion criteria can be found in the study protocol: TITAN-001, version 3.0, 2 July 2021 (Supplementary Information). Participants were reimbursed for transport expenses relating to the study and compensation for lost earnings during study visits but otherwise did not receive any financial compensation for participating in the study.
The sample size calculation was based on the primary endpoint: time to viral rebound during ATI. Time from stopping ART to loss of virological control was compared in the four randomization groups. If loss of virological control occurred, the date of the last measurement of plasma HIV-1 RNA ≥1,000 copies per milliliter or confirmed >100,000 copies per milliliter was defined as 'date of viral rebound'. Using a two-sample comparison of means with an s.d. of 11 d 1,4 , 10 evaluable participants in each of the two groups would have 90% power to detect a ≥16-d difference in time to viral rebound at a 5% significance level. To accommodate for dropouts, we aimed for 12 participants in each group. We considered a two-sided α value of less than 0.05 to be significant, with no adjustments made for multiple comparisons. We used the IPDA and d3PCR assays as our primary reservoir measurement, as intact proviral DNA is superior to total HIV-1 DNA in terms of estimating the intact HIV-1 reservoir 6 . Protocol amendments did not affect the analysis plan besides the reservoir size analyses described above.

Randomization
The Clinical Trial Unit at Aarhus University generated the randomization sequence using permuted blocks of four or eight by computer-generated random numbers without stratification to sex and age. Randomization assignment was provided to each site using REDCap.

Blinding
Participants, study physicians and nurses handling administrations of the study drugs, as well as those individuals doing the analyses, were blinded to interventions. Only the pharmacy and study personnel preparing the study drugs were unblinded to interventions.
The placebo for lefitolimod, 3BNC117 and 10-1074 was sterile physiological saline. Lefitolimod or placebo for lefitolimod was provided to the study physicians and nurses handling the injections as 4× 2-ml syringes. Placebo for 3BNC117 and 10-1074 or the bNAbs was administered by the study physicians and nurses handling the infusions as 250-ml piggy bags. Labeling contained only information of the study visit and an expiration date and time irrespective of either placebo or study drug.

Procedures
Lefitolimod or placebo was administered s.c. at a dose of 120 mg once weekly for the first 8 weeks from week −2 to week 5. Lefitolimod dosing was based on previously observed effects in clinical trials 12,13 . 3BNC117 (30 mg kg −1 ) and 10-1074 (20 mg kg −1 ) were sequentially administered as i.v. infusions over 60 min at weeks 0 and 3. The dosing of bNAbs was based on previously observed antiviral efficacy in clinical studies [27][28][29]34,44,60,61 . The infusions were performed in the Clinical Research Units at Aarhus University Hospital, Copenhagen University Hospital-Hvidovre or Rigshospitalet in Denmark; at the Clinical Research Section at the Department of Infectious Diseases, Oslo University Hospital in Norway; and in the Medical Day Unit at the Alfred Hospital, in Melbourne, Australia. Follow-up visits were conducted at the respective outpatient clinics of participating hospitals. End of study was defined as the timepoint for reaching criteria for viral rebound or week 25, whichever came first. Blood samples were collected at weeks −2, −1, 0, 1, 2, 3, 4, 5, 6,7,9,11,13,15,17,19,21,23 and 25 after ATI depending on when the participants reached criteria for viral rebound. Blood samples were processed within 4 h of collection, and serum and plasma samples were stored at −80 °C. PBMCs were isolated by density gradient centrifugation and cryopreserved (−150 °C) in FBS with 10% DMSO. Clinical safety assessments included directed physical examinations and vital sign measurement if indicated and review of AEs and concomitant medications at every visit and reported until the end of study (Fig. 1a). The Common Terminology Criteria for Adverse Events (CTCAE) version 5.0 grading scale was used to grade AEs. A safety monitoring committee was established to monitor the blinded safety data of the trial. Safety biochemistry was taken at weeks −2, 0, 3, 6, 9, 13 and 25 after ATI. CD4 + T cell counts were measured on weeks −2, 0, 1, 3, 5, 6, 9, 13, 17, 21 and 25. After resumption of ART, we monitored CD4 + T cell count every fourth week until plasma HIV-1 RNA levels were undetectable (<50 copies per milliliter).

25-week analytical treatment interruption
At week 0, participants were instructed to discontinue ART the following day and continue off ART for 25 weeks unless one of four criteria was met. Details can be found in the study protocol.

Plasma HIV-1 RNA measurements
Plasma HIV-1 RNA levels were measured with standardized clinical assays at every visit. After resumption of ART, we monitored plasma HIV-1 RNA every fourth week until levels were undetectable (<50 copies per milliliter).

Doubling time of plasma HIV-1 RNA
The initial increase in plasma HIV-1 RNA was calculated based on the first two consecutive plasma HIV-1 RNA measurements that increased, and the second measurement had to increase by 1,000 copies per milliliter compared to the previous measurement. The slope of this increase was calculated using a linear regression model. bNAb sensitivity bNAb sensitivity prediction was performed at screening for all participants primarily using the LabCorp-Monogram Biosciences Phe-noSense HIV Monoclonal Antibody Assay on proviral HIV-1 DNA. bNAb sensitivity was a pre-specified inclusion criterion. For the PhenoSense assay, sensitivity was determined based on the concentration of bNAb required to inhibit viral replication by 90% (IC 90 ). The sensitivity cutoffs were pre-defined as IC 90 <1.5 for 3BNC117 and <2.0 µg ml −1 for 10-1074 combined with a maximum percent inhibition (MPI) observed at the highest bNAb concentration tested ≥98%. If the PhenoSense assay did not yield a result (for example, due to insufficient amplification), env sequences obtained by single-genome amplification (SGA) of proviral HIV-1 DNA were secondarily analyzed using a genotypic prediction algorithm. For this genotypic assay, the pre-defined cutoff was >90% of sequences being sensitive to the bNAb for the proviral reservoir to be classified as 'sensitive'. If the env sequencing failed, the participant could still be enrolled based on the assumption of a lower HIV-1 reservoir size among these individuals (see the study protocol).

Proviral HIV-1 env sequencing
For screening of participants who failed the PhenoSense assay and post hoc for all participants receiving the bNAb combination, SGA, sequencing and genotypic analysis were performed on proviral HIV-1 env. DNA was isolated from PBMCs using the DNeasy Blood and Tissue Kit (Qiagen), and Platinum Taq DNA Polymerase (Invitrogen) was used for SGA (<30% of wells containing env). The env amplification was conducted in 10-µl reactions as nested PCRs. env amplification was, in most cases, first attempted with primers listed in the first box (Supplementary Table 4).
For participants in whom these primers did not amplify env, alternative primer sets and combinations were tested (second box, Supplementary Table 4). For a few of the participants, near full-length (NFL) HIV-1 sequencing was conducted to obtain sequences that could be used for individual env primer design. NFL amplification was conducted as SGA using Platinum Taq DNA Polymerase High Fidelity (Invitrogen) in 10-µl reactions. If the NFL sequences were resistant to one or both bNAbs, further analysis and amplification was not conducted.
All primer sequences, an overview of which primer set was used for each of the included participants and thermal cycler conditions can be found in Supplementary Table 4.
The env-positive wells were collected and prepared for sequencing 6 . In brief, tagmentation was conducted using TDE1 Tagment DNA Enzyme (Illumina) after ligation of barcoded primers using KAPA HiFi HotStart ReadyMix (Roche). The amplicons were pooled to one library, purified and paired-end sequenced on a MiniSeq (Illumina), using MiniSeq Mid-Output Kit (Illumina).

Rebound plasma HIV-1 env sequencing
Plasma rebound virus was sequenced for participants who received bNAbs and rebounded within the study period or were viremic at the end of the 25-week ATI. Extraction of RNA, cDNA synthesis and SGA of plasma HIV 6 , library preparation and sequencing were conducted as for proviral HIV-1 env sequencing.

HIV-1 sequence assembly and annotation
Assembly and annotation of HIV-1 sequences was performed by The Rockefeller University pipeline (Defective and Intact HIV Genome Assembler) 6 . The pipeline implements quality control checks to remove PCR-amplified reads, correct errors, trim adaptors and low-quality bases and remove potential contaminant reads. Paired overlapping reads are merged and subsequently used to reconstruct HIV-1 contigs. The longest reconstructed HIV-1 contig is annotated by aligning it to HXB2 reference. Finally, sequences not classified as double peaks (cutoff consensus identity for any residue <70%) are classified as intact or defective. Samples with <500 sequencing reads and sequences with double peaks were omitted from downstream analysis. Only intact HIV-1 envs were considered in the subsequent analysis.
Sequence-based assessment of bNAb sensitivity. The HIV-1 env sequences were analyzed for sensitivity to 3BNC117 and 10-1074 by searching for resistance mutations using a model based on sequencing and neutralization data (Supplementary Table 1).

Plasma antiretroviral drug concentrations
Antiretroviral drug concentrations in plasma were determined at BioXpedia using liquid chromatography with tandem mass spectrometry (LC-MS/MS) assay for individuals not fulfilling the criteria for viral rebound at week 13. Two timepoints were analyzed: (1) week 0 and (2) end of study, defined as the timepoint for reaching criteria for viral rebound or week 25, whichever came first.

Serum bNAb concentrations
Each bNAb concentration in human serum was quantitatively measured using a sandwich immunoassay on Meso Scale Discovery Electrochemiluminescence (MSD-ECL) platform at PPD Bioanalytical Lab. In this assay, the bNAb was captured by biotinylated anti-idiotypic anti-bNAb antibody coated onto an MSD streptavidin plate, which was then detected by SULFO-TAG-conjugated anti-idiotypic anti-bNAb antibody. The plate was read on an MSD plate reader, resulting in assay signal proportional to the concentration of each bNAb. The method was validated following current regulatory guidance, and a sensitivity of 100 ng m −1 was established for each pharmacokinetic assay. We measured the serum bNAb concentrations at weeks 0, 6, 13 and 25 after ATI.
We used multiple imputation to impute missing values in serum bNAb concentrations. A detailed description can be found in Supplementary Table 2.

Intact HIV-1 proviruses
The IPDA is used to measure intact and defective HIV-1 proviruses by targeting the Ψ and RRE, which are frequently deleted or mutated in defective proviruses 62 . We applied the IPDA protocol for all individuals regardless of subtype and found 20% (8/40) of the participants with Ψ, RRE or Ψ and RRE failure. For RRE failure, an alternative primer/probe set, reported to correlate with the original IPDA RRE primer/probe set, designed by Kinloch et al. 63 , was tested; this primer/probe set is located slightly downstream of the original IPDA RRE primer/probe set 63 . This alternative primer/probe set rescued all participants with RRE failure (n = 5) and worked for the participant with dual failure, thus leaving three participants with Ψ failure. For these three participants, the IPDA-like 3dPCR assay was applied, where individual primers and probes are designed for the participants based on sequencing of the participants'virus 6 . The level of intact proviruses measured by IPDA and 3dPCR were previously shown to strongly correlate in individuals with HIV-1 subtype B; thus, 3dPCR provides a reasonable estimate of intact HIV-1 reservoir size, although it is possible that the assay's positive predictive value for intact proviruses may, like the IPDA, vary between individuals 64 .
Regardless of the downstream assay applied-IPDA, IPDA with alternative RRE primer/probe or IPDA-like 3dPCR-CD4 + T cells (CD4 + T Cell Isolation Kit, Miltenyi Biotec) were isolated from 30 × 10 6 PBMCs, and genomic DNA was extracted (DNeasy Blood and Tissue Kit, Qiagen). IPDA, IPDA with alternative RRE primer/probe and the IPDA-like 3dPCR Article https://doi.org/10.1038/s41591-023-02547-6 were conducted 6,62,63 . An overview of the assay and primer/probe set used for each participant is provided in Supplementary Tables 3 and 4. In parallel with HIV-1 Ψ and RRE, all DNA extracts were also assayed in a separate duplexed ddPCR reaction targeting the human RPP30 gene 63 ; primers and probes are listed in Supplementary Table 5; and a detailed description of the method can be found in Supplementary Table 6.

HIV-1-specific T cell immunity
HIV-1-specific T cell immunity was assessed using the AIM assay by flow cytometry at weeks 0, 6, 13 and 25 after ATI. Aliquots of cryopreserved PBMCs were thawed, washed and rested at 37 °C for 3 h. Cells were then plated into wells of a 96-well plate at a total of 1 × 10 6 PBMCs per well in RMPI glutamine supplemented with penicillin-streptomycin and 10% FBS. Cells were then stimulated at a final concentration of 2 µg ml −1 of total peptide with four different HIV peptide pools for 20 h at 37 °C. Detailed description of the methods can be found in Supplementary Table 7, and the gating strategy can be found in Supplementary Fig. 1.

Cytokine detection
Cytokine detection of IFN-γ and GzmB were measured in supernatants from Gag and non-stimulated AIM assay using MSD U-PLEX Custom Immuno-Oncology (K151AEM-2) according to the manufacturer's instructions. The concentration of IFN-γ (pg ml −1 ) and GzmB (pg ml −1 ) in the supernatant of Gag-specific cells (AIM + cells) was determined by subtracting the concentration of the non-stimulation condition from the stimulated condition.

HLA class I typing
HLA class I (HLA-A, HLA-B and HLA-C) alleles were genotyped at the American Safety and Health Institute-accredited laboratory HistoGenetics using sequence-based typing.

Outcomes
The primary endpoint was time to loss of virological control during ATI (sustained plasma HIV-1 RNA ≥1,000 copies per milliliter for 4 weeks or confirmed plasma HIV-1 RNA >100,000 copies per milliliter). Secondary endpoints were (1) safety, including CD4 + T cell counts, and (2) viral kinetics during ATI: time to plasma HIV-1 RNA >50 and >1,000 copies per milliliter as well as doubling time of the initial increase in plasma HIV-1 RNA. Exploratory endpoints were (1) changes in reservoir size measured by intact HIV-1 proviruses and (2) effects on HIV-1-specific T cell immunity using the AIM assay.

Statistical methods
The analyses performed on primary, secondary and exploratory endpoints were pre-specified in the protocol. Paired two-tailed Wilcoxon tests and two-tailed Mann-Whitney tests were used to analyze non-parametric outcomes within and between groups, respectively. When more than two groups were compared, the Kruskal-Wallis test was used. Data are presented as median (IQR), median (range) or mean ± s.d. as indicated in each respective figure legend. The Kaplan-Meier estimator was used to assess the magnitude of the difference between the survival curves, and the log-rank test was used to compare time to loss of virologic control during ATI between groups. For correlations, Spearman's correlation coefficient was used. We used the full analysis set, comprising all individuals receiving at least one dose of active treatment with assessable data, for the efficacy analyses and all enrolled individuals for the safety analyses. We used Stata version 17.0 and Prism version 7.0 software for statistical analyses.

Reporting summary
Further information on research design is available in the Nature Portfolio Reporting Summary linked to this a rt ic le.

Data availability
Data are not available for download due to privacy/ethical restrictions under the European Union General Data Protection Regulation. Specific requests for access to the trial data may be sent to olesoega@ rm.dk, and access may be provided to a named individual in agreement with the rules and regulations (https://www.datatilsynet.dk/ english/legislation) of the Danish Data Protection agency and the Danish National Center for Ethics with a 2-week response timeframe for requests. All viral sequences have been deposited in GenBank with accession numbers OR014503 to OR015782 (www.ncbi.nlm.nih.gov/nucleotide/).

ART Resumed
Extended Data Fig. 4 | bNAb sensitivity at screening for the placebo/placebo and lefitolimod/placebo groups. bNAb sensitivity was primarily analyzed using the PhenoSense Monoclonal Antibody Assay with predefined IC90 thresholds for 3BNC117 ( < 1.5 µg/mL) and 10-1074 ( < 2.0 µg/mL) and maximum percentage inhibition (MPI) ≥ 98%. In seven participants, the PhenoSense Assay failed, and we secondarily used proviral HIV-1 envelope (env) sequences on a genotypic prediction algorithm with predefined thresholds of ≥90% known sequences are sensitive. Two participants (ID141 and ID313) were enrolled based upon the genotypically analysis, but post-hoc phenotypic data showed IC90 values of 3.69 and 3,59 µg/mL for 3BNC117, respectively. In another two participants (ID614 and ID808), both the PhenoSense Assay and env sequencing initially failed, and we tertiary-included (see Methods) these individuals based on the assumption that both assays failed due to a very small reservoir size. Empty red and blue triangles indicate placebo for 3BNC117 and 10-1074 infusions, respectively. Light grey shaded areas indicate time on ART, while white shaded boxes indicate still interrupting ART during the 25 weeks of ATI. Dark grey boxes are assay failures and pink boxes are no attempt to amplify. ART, antiretroviral therapy; ATI, ART interruption; env; HIV-1 envelope; IC90, concentration of bNAb required for 90% inhibition; i.v., intravenous.  Mean fraction of intact, 3′ and 5′ defective HIV-1 proviruses per 10 6 CD4 + T cells from 39 individuals at ATI start (day 0) (a). The size of the 3' (b) and 5' (c) defective HIV-1 reservoir at ATI start (day 0) and after 6, 13 and 25 weeks of ATI among individuals in the four randomization groups (lines at median and IQRs). Placebo/ placebo group (n = 10); lefitolimod/placebo (n = 9); placebo/bNAb (n = 10); and lefitolimod/bNAb (n = 11). All weeks are shown, including weeks with no data available. Median change in the 3' (d) and 5' (e) defective HIV-1 reservoir between week 6 of the ATI and pre-ATI for the four randomization groups are shown. Data are median and IQRs with the placebo/placebo group as the reference. Placebo/ placebo group (n = 10); lefitolimod/placebo (n = 8); placebo/bNAb (n = 9); and lefitolimod/bNAb (n = 9). P values comparing within groups (only P values below 0.05 are shown) and between groups were calculated using the paired two-tailed Wilcoxon test and two-tailed Mann-Whitney test, respectively. ART, antiretroviral therapy; ATI, ART interruption; IQR, interquartile ranges; bNAb, broadly neutralizing antibody.  Fig. 7 | CD4 + T cell count at trial start and end-of-study.