Extended Data Fig. 5: IHC and quantification of OA/MF with factors at 4 weeks. | Nature Medicine

Extended Data Fig. 5: IHC and quantification of OA/MF with factors at 4 weeks.

From: Articular cartilage regeneration by activated skeletal stem cells

Extended Data Fig. 5

a, Representative IF of (from left-right) ACAN, COL 1, COL 2, COL 10 and MMP 13 in 3 treatment conditions (top: PBS, middle: BMP2, bottom: BMP2 + sVEGFR1) at 4 weeks after MF. Scale bars 250μm. (n = 8 per group). b, Quantification of IF stains for indicated markers of MF + factors at 4 weeks. Graphs show mean + /- s.e.m. Ordinary one-way ANOVA test with post-hoc analysis using Šídák method to compare between specific means. Exact P values to 2 significant figures. c, Representative IF isotype and positive controls. (n = 3 per group). d, Repeat examples of pentachrome staining of BMP2 + sVEGFR1 sections at low (scale bars 500μm) and high (scale bars 100μm) magnification. Representatives of eight independent experiments.

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