Table 3 RT–PCR assay for detection of SARS-Co-2 viral load with QuantiNova Probe RT–PCR kit

From: Infection of bat and human intestinal organoids by SARS-CoV-2

Primer/probe sequence
F: 5′-CGCATACAGTCTTRCAGGCT-3′
R: 5′-GTGTGATGTTGAWATGACATGGTC-3′
Pb: 5′ FAM-TTAAGATGTGGTGCTTGCATACGTAGAC -lABkFQ-3′
ComponentsVolume (μl)
2× buffer10
Water1.2
RT mix0.2
Forward primer (10 μM)1.6
Reverse primer (10 μM)1.6
Probe (10 –M)0.4
Subtotal15
RNA5
Cycling profile
TemperatureTimeCycle
45 °C10 min1
95 °C5 min1
95 °C5 s45
55 °C30 s45
40 °C30 s1