a, Culture media were harvested from the infected bat enteroids and subjected to viral load detection and viral titration by TCID50 assay. Data represent the results of a representative experiment, n = 2. Independent experiments were performed three times in duplicate or triplicate. b, SARS-CoV-2-infected or mock-infected bat enteroids were fixed and immunostained to identify viral NP (green)-positive cells. Nuclei and actin filaments were counterstained with DAPI (blue) and Phalloidin-647 (purple), respectively. c, Differentiated bat enteroids were fixed and immunostained using antibodies against human homologues to show ACE2 (green) and TMPRSS2 (green) protein. ACE2 is shown alone (left) and merged with actin (right). The organoids stained with the second antibody only are imaged as a control (Ctrl). Scale bar, 10 µm. Images are representatives of at least five similar images.