While polygenic risk scores (PRSs) are poised to be translated into clinical practice through prediction of inborn health risks1, a strategy to utilize genetics to prioritize modifiable risk factors driving heath outcome is warranted2. To this end, we investigated the association of the genetic susceptibility to complex traits with human lifespan in collaboration with three worldwide biobanks (ntotal = 675,898; BioBank Japan (n = 179,066), UK Biobank (n = 361,194) and FinnGen (n = 135,638)). In contrast to observational studies, in which discerning the cause-and-effect can be difficult, PRSs could help to identify the driver biomarkers affecting human lifespan. A high systolic blood pressure PRS was trans-ethnically associated with a shorter lifespan (hazard ratio = 1.03[1.02–1.04], Pmeta = 3.9 × 10−13) and parental lifespan (hazard ratio = 1.06[1.06–1.07], P = 2.0 × 10−86). The obesity PRS showed distinct effects on lifespan in Japanese and European individuals (Pheterogeneity = 9.5 × 10−8 for BMI). The causal effect of blood pressure and obesity on lifespan was further supported by Mendelian randomization studies. Beyond genotype–phenotype associations, our trans-biobank study offers a new value of PRSs in prioritization of risk factors that could be potential targets of medical treatment to improve population health.
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The genotype data of BBJ used in this study are available from the Japanese Genotype-phenotype Archive (http://trace.ddbj.nig.ac.jp/jga/index_e.html) with the accession code JGAD00000000123. The GWAS summary statistics for BBJ are available at the National Bioscience Database Center Human Database with the accession code hum0014. The UKB analysis was conducted via the application 31063, and its GWAS summary statistics are available at http://www.nealelab.is/uk-biobank. This study used the FinnGen release 3 data. Summary statistics from FinnGen are available on request from the FinnGen project and are being prepared for public release in May 2020.
We used publicly available software for the analyses. The software programs used are listed and described in the Methods.
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We thank all the participants of BBJ, UKB and FinnGen. We thank A. Palotie for his support for the data analysis of FinnGen, B. M. Neale and N. Baya for sharing and discussing their idea on LOGO, and A. R. Martin for the PRS analysis on UKB. We thank K. Yamamoto for supporting our analyses. This research was supported by the Tailor-Made Medical Treatment Program (the BBJ Project) of the Ministry of Education, Culture, Sports, Science, and Technology (MEXT), the Japan Agency for Medical Research and Development (AMED). The FinnGen project is funded by two grants from Business Finland (HUS 4685/31/2016 and UH 4386/31/2016) and nine industry partners (AbbVie, AstraZeneca, Biogen, Celgene, Genentech, GSK, MSD, Pfizer and Sanofi). The following biobanks are acknowledged for collecting the FinnGen project samples: Auria Biobank (https://www.auria.fi/biopankki/), THL Biobank (https://thl.fi/en/web/thl-biobank), Helsinki Biobank (https://www.terveyskyla.fi/helsinginbiopankki/), Northern Finland Biobank Borealis (https://www.ppshp.fi/Tutkimus-ja-opetus/Biopankki), Finnish Clinical Biobank Tampere (https://www.tays.fi/biopankki), Biobank of Eastern Finland (https://ita-suomenbiopankki.fi), Central Finland Biobank (https://www.ksshp.fi/fi-FI/Potilaalle/Biopankki), Finnish Red Cross Blood Service Biobank (https://www.bloodservice.fi/Research%20Projects/biobanking), Terveystalo Biobank Finland (https://www.terveystalo.com/fi/Yritystietoa/Terveystalo-Biopankki/Biopankki/). Y.O. was supported by the Japan Society for the Promotion of Science (JSPS) KAKENHI (15H05911, 19H01021), AMED (JP19gm6010001, JP19ek0410041, JP19ek0109413, and JP19km0405211), the Takeda Science Foundation, and the Bioinformatics Initiative of Osaka University Graduate School of Medicine, Osaka University. M.Kanai was supported by a Nakajima Foundation Fellowship and the Masason Foundation.
The authors declare no competing interests.
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Hazard ratios (HRs) from Cox proportional-hazard models for lifespan according to observed phenotypes in BioBank Japan (BBJ [n=179,066]; a) and UK Biobank (UKB [n=361,194]; b) are shown and compared. The boxes indicate the point estimates, and the horizontal bars indicate the 95% confidence interval. Boxes colored in blue (a) or green (b) indicate the nominal significance (P < 0.05) and the white-filled boxes indicate the statistical significance after correcting for multiple testing by the Bonferroni method. All the acronyms are described in Fig. 2.
Extended Data Fig. 2 The relationship between systolic blood pressure and HR for age at death in BioBank Japan.
The HR for age at death according to the observed systolic blood pressure in BBJ (n=179,066) is shown. The dotted lines represent the 95% confidence interval.
The results of hazard ratios from sex-stratified Cox proportional-hazard models for lifespan, according to the PRS of the clinical phenotypes in (a) BioBank Japan (n=179,066), (b) UK Biobank (n=361,194), and (c) FinnGen (n=135,638) are shown. The boxes in blue indicate the point estimates in males, and those in red indicate the point estimates in females. The horizontal bars are the 95% confidence interval. For both sexes, we separately performed the fixed-effect meta-analyses of the association results from the three cohorts (d) by the inverse-variance method.
Shown are the results of two-sample Mendelian randomization studies with an inverse-variance weighted method to estimate the causal effect of biomarkers on lifespan in (a) BioBank Japan (n=179,066), (b) UK Biobank (n=361,194), and (c) FinnGen (n=135,638). We performed a fixed-effect meta-analysis of the association results from the three cohorts (d) by the inverse-variance method (ntotal=675,898), and displayed only nominally significant traits (9 out of 33 investigated traits). The circles indicate the point estimates, and the horizontal bars are the 95% confidence interval. Circles in colors indicate the nominal significance (P < 0.05) and the white-filled circles indicate the statistical significance after the Bonferroni correction for multiple testing. The size of the circles reflects the statistical significance in -log10(P).
Extended Data Fig. 5 The overlap of the variants constituting the PRSs between UK Biobank and BioBank Japan.
a, Among the variants constituting UK Biobank PRSs, the variants in blue did not exist in BioBank Japan variant dataset, those in green existed in BioBank Japan variant dataset, and those in pink were shared with or tagged (r2 > 0.8) by the variants constituting BioBank Japan PRSs of the same trait. To calculate r2 of LD, we used the LD reference panel from 5,000 randomly selected BioBank Japan individuals. Please note that the variants constituting PRSs from all the 10 sub-groups were concatenated in 20 traits with LOGO analysis. b, Among the variants constituting BioBank Japan PRSs, the variants in blue did not exist in UK Biobank variant dataset, those in green existed in UK Biobank variant dataset, and those in pink were shared with or tagged by the variants constituting UK Biobank PRSs of the same trait. To calculate r2 of LD, we again used the LD reference panel from 5,000 randomly selected BioBank Japan individuals. Please note that the variants constituting PRSs from all the 10 sub-groups were concatenated in all 33 traits with LOGO analysis.
Extended Data Fig. 6 A funnel plot for the effects of systolic blood pressure (sBP) PRS on lifespan, according to disease groups.
Sensitivity analyses of the effect of sBP PRS on the age at death. A funnel plot of the effects of sBP PRS on the age at death is shown by stratifying study participants into disease groups with at least 3,000 case samples (ntrait=22). The effect sizes from Cox proportional-hazard models are on x axis, and inverse standard errors (precision) are on y axis. A dotted line indicates the effect size from overall participants (n=179,066).
A distribution of normalized sBP PRS and the stratification according to the quintiles. We defined the lowest, intermediate, and highest PRS bins according to the quintiles of PRS (first, 2-4th, and fifth, respectively). Each quintile bin was defined so as to have the same number of participants.
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Sakaue, S., Kanai, M., Karjalainen, J. et al. Trans-biobank analysis with 676,000 individuals elucidates the association of polygenic risk scores of complex traits with human lifespan. Nat Med (2020). https://doi.org/10.1038/s41591-020-0785-8