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IMMUNOTHERAPY

Using single-cell analysis to predict CAR T cell outcomes

Nature Medicine volume 26, pages 1813–1814 (2020)Cite this article

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Single-cell analysis provides novel insights into elements of CAR T cell toxicities and response.

CAR T cells are T cells genetically engineered to combine the extracellular domain of antibody single-chain variable fragment with the intracellular domain of T cells signaling; this therefore merges antibody-based specificity with T cell cytotoxicity to allow major histocompatibility complex–independent targeting of tumor antigens6. CAR T cells are typically generated from lymphocytes derived from autologously collected apheresis samples; thus, the composition of CAR T cells is uniquely personalized to the patient and incorporates vast heterogeneity, which may contribute to the wide presentation of cytokine-release syndrome, neurotoxicity (immune effector cell–associated neurotoxicity syndrome) and CAR T cell efficacy.

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Fig. 1: Understanding the diversity of responses to CD19 CAR T cells.

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Authors and Affiliations

  1. Division of Pediatric Hematology/Oncology, Department of Pediatrics, Stanford University, Palo Alto, CA, USA

    Sneha Ramakrishna

  2. Pediatric Oncology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA

    Nirali N. Shah

Authors
  1. Sneha Ramakrishna
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  2. Nirali N. Shah
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Correspondence to Nirali N. Shah.

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The authors declare no competing interests.

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Ramakrishna, S., Shah, N.N. Using single-cell analysis to predict CAR T cell outcomes. Nat Med 26, 1813–1814 (2020). https://doi.org/10.1038/s41591-020-01157-w

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  • DOI: https://doi.org/10.1038/s41591-020-01157-w

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