Fig. 3: AIM assay evaluation of T cell responses to multiple HIV-1 antigens. | Nature Medicine

Fig. 3: AIM assay evaluation of T cell responses to multiple HIV-1 antigens.

From: Combination anti-HIV-1 antibody therapy is associated with increased virus-specific T cell immunity

Fig. 3

AIM+ T cells include cells that were PD-L1+CD69+ or 4-1BB+CD69+ or PD-L1+4-1BB+. a, Net frequency of HIV-1 Gag-specific AIM+CD8+ (left, red) and CD4+ T cells (right, blue) for each bNAb + ATI individual. Net frequency of the Gag-stimulated condition was calculated by subtracting the frequency detected in a DMSO control. b, Comparison of net frequency HIV-1 Gag-specific AIM+CD8+ (left, blue) and CD4+ T cells (right, red) at week −2 and week 12. Symbols represent biologically independent samples from n = 9 bNAb + ATI individuals. Lines connect data from the same donor. Bars show median values. c, Comparison of the frequency of Gag-specific CD8+ or CD4+ T cells identified by AIM assay or ICS. The or-gate strategy was used for both assays. Symbols represent biologically independent samples from n = 9 bNAb + ATI individuals (samples obtained at week −2 and 12 for each individual were included for comparison). Lines connect data from the same donor and time point. d, Relationship between the frequency of Gag-specific CD8+ and CD4+ T cell responses identified either by AIM assay or ICS. Symbols represent biologically independent samples from n = 9 bNAb + ATI individuals (samples obtained at week −2 and 12 for each individual were included for comparison). Association was determined by Spearman correlation. e, Net frequency of HIV-1 Pol, Nef, gp120 or gp41-specific CD8+ (upper graphs) or CD4+ T cells (lower graphs) identified by AIM assay in bNAb + ATI individuals at week −2 and week 12. Symbols represent biologically independent samples from n = 9 bNAb + ATI individuals. Lines connect data from the same donor. Median values are shown as bars. P values indicated in b,c,e were calculated by a paired two-tailed Wilcoxon test.

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