HIV-1-specific T cell responses identified by PD-L1/CD69/4-1BB AIM assay were analyzed for surface expression of HLA-DR/CD38 and PD-1. (a) Representative plot showing expression of HLA-DR/CD38 and PD-1 on AIM+ HIV-1-specific CD8+ (upper graphs) and CD4+ T cells (lower graphs). Flow panels are representative of n=6 (CD8+) or n=8 (CD4+) biologically independent bNAb+ATI individuals. (b) Frequency of HLA-DR+CD38+ or PD-1+ of HIV-1-specific CD8+ and CD4+ T cell responses at week -2 or week 12. Symbols represent biologically independent samples from n=6 (CD8+) and n=8 (CD4+) bNAb+ATI individuals. Only samples with AIM-responses that are at least 2-fold over DMSO-stimulated control condition were analyzed for phenotype to limit the contribution of background events. Lines connect data from the same donor. Bars show median values. P values are indicated in graphs and were calculated by paired two-tailed Wilcoxon test.