Extended Data Fig. 10: Dose-response and in silico analysis of PEP005 and related molecules. | Nature Medicine

Extended Data Fig. 10: Dose-response and in silico analysis of PEP005 and related molecules.

From: iPSC modeling of young-onset Parkinson’s disease reveals a molecular signature of disease and novel therapeutic candidates

Extended Data Fig. 10

(a) Structures of Phorbol esters similar to PEP005 tested in mDA cultures. (b) Western blots of α-synuclein (α-Syn) and p-PKCα in response to varying PEP005 doses. (c) Western blots of α-Syn and p-PKCα in response to varying Prostratin (PRO) doses in both YOPD and control mDA cultures. Dose ranging studies were repeated twice. (d) Predictive modeling of PEP005 binding sites on PKCα and similar affinity sites on additional proteins. (e) Three dimensional model of PEP005 binding sites on PKCα, PKCδ, and Ras overlaid to show similarity.

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