(a) Tyrosine Hydroxylase (TH) production by western blot across 8 control and 12 YOPD patients. TH levels were first normalized to β-actin, then to compare across blots the levels were normalized to the average signal (TH/ β-actin) of each gel. (b) Western blots of TH and α-synuclein (α-Syn) levels in day 30 mDA cultures across 3 unique clonal lines from ED044iCTR and from 192iPD. (c) Quantification of band intensities for TH and α-Syn normalized to β-actin. Bands represent independent biological replicates from 3 separate wells differentiated in the same experiment. *indicates p=0.0002 via one-way ANOVA (F 51.42, DF 8) with Tukey multiple comparisons test compared to other clones of the same line. Bar graphs represent mean, error bars represent standard deviation (s.d.).