Extended Data Fig. 8: Additional characterization of PEP005 treatment. | Nature Medicine

Extended Data Fig. 8: Additional characterization of PEP005 treatment.

From: iPSC modeling of young-onset Parkinson’s disease reveals a molecular signature of disease and novel therapeutic candidates

Extended Data Fig. 8

(a) Immunocytochemistry showing TH and α-synuclein (α-Syn) in 200iPD d30 mDA cultures with and without PEP005 treatment. Images are representative of 2 additional lines tested. (b) Day 30 mDA neurons treated with PEP005 from multiple YOPD and control lines. (c) Quantification of LC3I/II and α-Syn band intensities relative to untreated cells from the same line. (d) Time-course of PEP005 treatment in YOPD and control mDA neurons. (e) Quantification of α-Syn, p-PKCα, LAMP1, and TH band intensities in YOPD and control mDA neurons in timecourse study. qPCR from paired samples (n=3 wells 02iCTR n=3 wells 190iPD) over PEP005 time-course showing (f) SNCA and (g) TH expression. Bar graphs represent mean, error bars represent standard deviation (s.d.).

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