Extended Data Fig. 5: Significant differentiation in PEG hydrogels leads to lower fold expansion of CD34+ cells after 24 d. | Nature Medicine

Extended Data Fig. 5: Significant differentiation in PEG hydrogels leads to lower fold expansion of CD34+ cells after 24 d.

From: Expansion of primitive human hematopoietic stem cells by culture in a zwitterionic hydrogel

Extended Data Fig. 5

a, The modulus of ZTG and PEG hydrogels at day 0. b, The modulus loss of ZTG and PEG hydrogels at day 14. c, Percentage of CD34+ cells after culture in ZTG and PEG hydrogels for 14 and 24 d. d, Fold expansion of CD34+ cells after culture in ZTG and PEG hydrogels for 14 and 24 d. e, Dissolved oxygen (DO) levels measured after 24-h culture in each system. f, MFI of cellular ROS level in each culture at culture day 1. Values represent mean ± s.d., n = 3 biologically independent experiments and the two-tailed Student’s t-test P values are indicated.

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