Extended Data Fig. 1: CD19 scFvs derived from different CD19 hybridomas show different kinetic binding properties. | Nature Medicine

Extended Data Fig. 1: CD19 scFvs derived from different CD19 hybridomas show different kinetic binding properties.

From: Enhanced CAR T cell expansion and prolonged persistence in pediatric patients with ALL treated with a low-affinity CD19 CAR

Extended Data Fig. 1

Anti-CD19 scFvs were fused to mouse IgG2a-Fc in a SFG-eBFP γ-retroviral expression vector and HEK293T cells were transfected to generate secreted scFvs, which were then purified on a protein A column. a, Surface plasmon resonance sensograms of binding kinetics between CAT and FMC63 scFv-Fcs to CD19. b, Tabulated kinetic and equilibrium dissociation constants of the CD19 binders. Experiments were repeated independently twice with similar results.

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