a–d. Analysis of sIgA+ (a,b) and IgE+ (c,d) fecal bacteria in OVA/SEB-sensitized Foxp3YFPCre, Il4raF709Foxp3YFPCre and Foxp3YFPCreRorcΔ/Δ mice. Fecal pellets from Rag2–/– and Igh7–/–Il4raF709 mice were used as negative controls: Foxp3YFPCre (n = 6 per group), Il4raF709Foxp3YFPCre (n = 11 and 7) and Foxp3YFPCreRorcΔ/Δ mice (n = 10 and 8). e–f, Analysis of GATA3+Foxp3+ Treg cells in the following OVA/SEB-sensitized mice that were either untreated or treated with Clostridiales or Bacteroidales consortium: Il4raF709Foxp3YFPCre (n = 9, 5 and 5) and Il4raF709Foxp3YFPCreRorcΔ/Δ (n = 4, 5 and 8). g,h, Analysis of GATA3+Foxp3+ Treg cells in OVA/SEB-sensitized Il4raF709Foxp3YFPCre mice treated with the Bacteroidales consortium (n = 9), and in OVA/SEB-sensitized Il4raF709Foxp3YFPCreMyd88Δ/Δ mice otherwise untreated or treated with the Clostridiales or Bacteroidales consortium (n = 8, 9 and 8). Each symbol represents one mouse. Results represent means ± s.e.m. collated from two independent experiments. P values were derived by one-way ANOVA with Dunnett’s post hoc analysis (b,d,f,h).