Extended Data Fig. 8: Oral SCFA supplementation does not protect against FA. | Nature Medicine

Extended Data Fig. 8: Oral SCFA supplementation does not protect against FA.

From: Microbiota therapy acts via a regulatory T cell MyD88/RORγt pathway to suppress food allergy

Extended Data Fig. 8

a, SCFA in fecal samples of PBS or OVA/SEB-sensitized WT and Il4raF709 mice. Acetate, propionate and butyrate: WT, PBS or OVA/SEB (n = 5 per group); Il4raF709, PBS (n = 5 per group) or OVA/SEB (n = 10 per group). Isovalerate: WT, PBS or OVA/SEB (n = 5 and 4); Il4raF709, PBS or OVA/SEB (n = 5 and 8). Valerate: WT, PBS or OVA/SEB (n = 5 and 3); Il4raF709, PBS or OVA/SEB (n = 4 and 7). b, Temperature changes in OVA-challenged WT and Il4raF709 mice sensitized with PBS or OVA/SEB without or with SCFA supplementation: WT, PBS+SCFA (n = 10), WT, OVA/SEB (n = 11), WT, OVA/SEB+SCFA (n = 24); Il4raF709, PBS+SCFA (n = 12), Il4raF709, OVA/SEB (n = 7), Il4raF709, OVA/SEB+SCFA (n = 17). c, Total and OVA-specific IgE: WT, PBS+SCFA (n = 5 per group), WT, OVA/SEB (n = 6 per group), WT, OVA/SEB+SCFA (n = 9 per group); Il4raF709, PBS+SCFA (n = 4 per group), Il4raF709, OVA/SEB (n = 8 per group), Il4raF709, OVA/SEB+SCFA (n = 9 per group). d, Frequencies of MLN CD4+Foxp3+ROR-γt+ and CD4+Foxp3ROR-γt+ T cells: WT, PBS+SCFA (n = 5 per group), WT, OVA/SEB (n = 4 per group), WT, OVA/SEB+SCFA (n = 4 per group); Il4raF709, PBS+SCFA (n = 5 per group), Il4raF709, OVA/SEB (n = 5 per group), Il4raF709, OVA/SEB+SCFA (n = 7 per group). Each dot represents one mouse. Data represent mean ± s.e.m. from two independent experiments. P values were derived by the Kolmogorov–Smirnov test (a), by Student’s unpaired two-tailed t-test (c,d) or by two-way ANOVA (b).

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