Extended Data Fig. 6: A Bacteroidales consortium prevents FA. | Nature Medicine

Extended Data Fig. 6: A Bacteroidales consortium prevents FA.

From: Microbiota therapy acts via a regulatory T cell MyD88/RORγt pathway to suppress food allergy

Extended Data Fig. 6

a, Left: experimental schema; right: temperature changes in GF Il4raF709 mice that were colonized and sensitized as indicated then challenged with OVA (n = 5 per group). b,c, Total and OVA-specific IgE (b) and MMCP-1 concentrations (c). GF, OVA/SEB (n = 5 per group), Bacteroidales, PBS (n = 6, 7 and 7), Bacteroidales, OVA/SEB (n = 6, 6 and 7). d, Frequencies of MLN CD4+Foxp3+, IL-4+Foxp3+ and GATA3+Foxp3+ T cells. GF, OVA/SEB (n = 5 per group), Bacteroidales, PBS (n = 4, 8 and 5), Bacteroidales, OVA/SEB (n = 6, 5 and 6). e, Frequencies of HeliosNrp1Foxp3+ and ROR-γt+Foxp3+ T cells. GF, OVA/SEB (n = 5 and 7), Bacteroidales, PBS (n = 5 per group), Bacteroidales, OVA/SEB (n = 6 per group). f, Left: experimental schema; right: temperature changes in Il4raF709 mice sensitized and treated as indicated. OVA/SEB (n = 6), OVA/SEB, Bacteroidales, (n = 5). g, Total and OVA-specific IgE and MMCP-1 concentrations: OVA/SEB (n = 7, 9 and 9), OVA/SEB, Bacteroidales, (n = 5, 10 and 5). h,i, Frequencies of MLN CD4+Foxp3+, IL-4+Foxp3+ and GATA3+Foxp3+ (h) and HeliosNrp1Foxp3+ and ROR-γt+Foxp3+ T cells (i). OVA/SEB (n = 5, 8 and 3, 5 and 5), OVA/SEB, Bacteroidales (n = 5, 10, 4, 6 and 9). j, IgE and IgA staining of fecal bacteria. OVA/SEB (n = 11 per group), OVA/SEB, Bacteroidales (n = 8 per group). Each dot represents one mouse. Data represent mean ± s.e.m. from two independent experiments. P values were derived by repeat measures two-way ANOVA (a,f), by one-way ANOVA with Dunnett’s post hoc analysis (be) or by Student’s unpaired two-tailed t-test (hj).

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