Extended Data Fig. 8: Gating strategies for antibody-dependent phagocytosis, antibody-dependent neutrophil phagocytosis and NK cell activation. | Nature Medicine

Extended Data Fig. 8: Gating strategies for antibody-dependent phagocytosis, antibody-dependent neutrophil phagocytosis and NK cell activation.

From: IFN-γ-independent immune markers of Mycobacterium tuberculosis exposure

Extended Data Fig. 8

a, Antibody-dependent cellular phagocytosis of PPD and or ESAT6/CFP10 adsorbed fluorescent FITC beads was measured in THP-1 monocytes. After gating on size, granularity and singlets, the frequency and mean fluorescence intensity of FITC beads was measured. The phagocytic score was calculated as the integrated MFI (percentage frequency × MFI/10,000)50. b, Antibody-dependent neutrophil phagocytosis of PPD adsorbed fluorescent FITC beads was measured. Neutrophils were identified by CD66b staining after gating on size, granularity and singlets. The frequency and mean fluorescence intensity of FITC beads was measured, and phagocytic score was calculated as the integrated MFI (percentage frequency × MFI/10,000). c, For antibody-dependent NK-cell activation, cells were first captured after gating on size, granularity and singlets. CD3+ T lymphocytes were gated out and CD16 was used to identify NK cells for which MIP-1β expression, and CD107a and IFN-γ production were measured.

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