Extended Data Fig. 4: ‘Resisters’ are not globally deficient in IFN-γ production by T cells. | Nature Medicine

Extended Data Fig. 4: ‘Resisters’ are not globally deficient in IFN-γ production by T cells.

From: IFN-γ-independent immune markers of Mycobacterium tuberculosis exposure

Extended Data Fig. 4

a, The COMPASS heatmap shows 49 informative CD4 T cell subset responses to staphylococcus enterotoxin B (SEB) for n = 42 individuals. Rows represent study subjects and columns represent CD4 T cell functional subsets. The depth of shading within the heatmap represents the probability of detecting a response to a given subset in a given subject above background. IFN-γ-containing subsets are noted in red. b, Subject-specific COMPASS results in response to SEB stimulation were summarized using a polyfunctionality score, which weights T cell subsets that include more than one function. Boxplots show median and interquartile range. Statistical significance was calculated by Mann–Whitney U test and the two-tailed P value is shown. c, Representative flow cytometry plots from a ‘resister’ and LTBI subject show frequencies of CD154+IFN-γ+IL-2+TNF+ T cells (red dots) in response to stimulation with SEB or DMSO, with each experiment performed once. d, The COMPASS heatmap shows 10 informative CD8 T cell subset responses to CMV, Epstein Barr virus (EBV) and influenza (CEF) combined peptide pool. IFN-γ containing subsets are noted in red. e, Polyfunctionality scores are depicted for n = 21 RSTRs and n = 21 LTBI subjects with boxplots show median and interquartile ranges. Statistical significance was calculated by Mann–Whitney U test and the two-tailed P value is shown. f, Representative flow cytometry plots from a ‘resister’ and LTBI subject display nearly equivalent frequencies of CD107a+IFN-γ+IL-2+TNF+ T cells (red dots) in response to stimulation with CEF or DMSO, with each experiment performed once.

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