A 15-year-old patient with cystic fibrosis with a disseminated Mycobacterium abscessus infection was treated with a three-phage cocktail following bilateral lung transplantation. Effective lytic phage derivatives that efficiently kill the infectious M. abscessus strain were developed by genome engineering and forward genetics. Intravenous phage treatment was well tolerated and associated with objective clinical improvement, including sternal wound closure, improved liver function, and substantial resolution of infected skin nodules.
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We thank T. Sampson, L. Holst, and S. Pinches for the discovery of BPs, Muddy, and ZoeJ, respectively, and the students and faculty in the Mycobacterial Genetics Course at the University of KwaZulu Natal and in the SEA-PHAGES program for their contributions in isolating and characterizing the collection of phages used here. Further information about the phages, who isolated them and from where is available at https://phagesdb.org. We thank the Great Ormond Street Hospital staff in the Departments of Microbiology, Cell Therapy, and Pharmacy for excellent technical assistance, C. Murphy for assistance with strains and sera, J. Standing and B. Margetts for assistance with figures, D. Bain at the University of Pittsburgh for the ICP-MS analysis, and A. Betsko for electron microscopy. We greatly appreciate the advice of J. Hartley on regulatory aspects, and thank C. Hamilton for help with translation. We thank T. Mavrich for comments on the manuscript and we are grateful to S. Strathdee for general advice and comments on the manuscript. This work was funded by the National Institutes of Health (grant GM116884 to G.F.H.) and the Howard Hughes Medical Institute (grant 54308198 to G.F.H.).
R.T.S. serves as an uncompensated member of the AmpliPhi Scientific Advisory Board. Other authors declare no competing interests.
Publisher’s note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Timeline showing administration of antibiotics, immunosuppressive drugs, and the phage cocktail. Levels of the immunosuppressive drug Tacrolimus are shown at the top, and the administration of drugs is as indicated.
Genes are shown as colored boxes above or below a genome track, reflecting rightwards and leftwards transcription, respectively. Pairwise nucleotide sequence similarity is indicated by spectrum-colored shading between genomes, with violet representing closest similarity.
a, Approximately 5 × 108 cells of M. abscessus GD01 in one ml were incubated with a cocktail of 109 pfu each of three phages for one week in liquid culture. Aliquots (100 µl) were plated onto solid media and incubated at 37 °C. In the absence of phage, a confluent lawn grew (left), and in the presence of phage (right), approximately 150 small colonies were observed. b, Six individual colonies were picked, grown and retested for phage susceptibilities. Top agar overlays with each strain were plated on solid media and 10-fold serial dilutions of phages (as indicated) were spotted onto each plate.
Phage preparations of Muddy, ZoeJΔ45, and BPs33ΔHTH-HRM10 (as shown) each containing approximately 2 × 1010 phage particles were separated by SDS-PAGE, together with protein markers (M) and a control sample of 10 µl of a 1:100 dilution of patient serum (serum) collected 72 days after initiation of phage treatment. The gel was stained with Coomassie Blue (left), transferred to a membrane for a Western blot which was probed with the same patient serum and an anti-human Horse Radish Peroxidase conjugated secondary antibody. These assays were repeated three times with similar results; a representative experiment is shown.
M. abscessus were recovered at 20-, 72-, 107, and 121-days after initiation of phage treatment, propagated, and tested for susceptibilities to each of the phages in the cocktail. Each phage was diluted serially 10-fold and spotted onto bacterial lawns. These assays were repeat at least twice with similar results; a representative experiment is shown.
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Dedrick, R.M., Guerrero-Bustamante, C.A., Garlena, R.A. et al. Engineered bacteriophages for treatment of a patient with a disseminated drug-resistant Mycobacterium abscessus. Nat Med 25, 730–733 (2019). https://doi.org/10.1038/s41591-019-0437-z
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