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Robust prediction of response to immune checkpoint blockade therapy in metastatic melanoma

Matters Arising to this article was published on 05 December 2019

A Publisher Correction to this article was published on 17 October 2018

This article has been updated


Immune checkpoint blockade (ICB) therapy provides remarkable clinical gains and has been very successful in treatment of melanoma. However, only a subset of patients with advanced tumors currently benefit from ICB therapies, which at times incur considerable side effects and costs. Constructing predictors of patient response has remained a serious challenge because of the complexity of the immune response and the shortage of large cohorts of ICB-treated patients that include both ‘omics’ and response data. Here we build immuno-predictive score (IMPRES), a predictor of ICB response in melanoma which encompasses 15 pairwise transcriptomics relations between immune checkpoint genes. It is based on two key conjectures: (i) immune mechanisms underlying spontaneous regression in neuroblastoma can predict melanoma response to ICB, and (ii) key immune interactions can be captured via specific pairwise relations of the expression of immune checkpoint genes. IMPRES is validated on nine published datasets1,2,3,4,5,6 and on a newly generated dataset with 31 patients treated with anti-PD-1 and 10 with anti-CTLA-4, spanning 297 samples in total. It achieves an overall accuracy of AUC = 0.83, outperforming existing predictors and capturing almost all true responders while misclassifying less than half of the nonresponders. Future studies are warranted to determine the value of the approach presented here in other cancer types.

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Fig. 1: Shared immune components between melanoma and neuroblastoma.
Fig. 2: Response and survival predictive performance of IMPRES.
Fig. 3: Comparative performance of IMPRES and its underlying features.

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The authors thank M. Leiserson, S. Patkar, E. Persi, W. Robinson, Z. Ronai, Y. Saadon, Y. Samuels and J. Wherry for their helpful comments. N.A., J.S.L. and E.R. were partially supported by a grant from the Israeli Science Foundation (ISF, grant no. 41/11) and R33-CA225291-01. E.R. is supported by the intramural program at the CCR, NCI. N.A. is supported by the NCI–UMD partnership for integrative cancer research. M.H. and K.F. are supported by the Dr. Miriam and Sheldon G. Adelson Medical Research Foundation. M.H. is supported by NIH grants 5P01CA114046, 5P50CA174523 and 1U54CA224070, and the Peer Reviewed Cancer Research Program Grant WX1XWH-16-1-0119 [CA150619].

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Authors and Affiliations



E.R. supervised the research. N.A. and E.R. conceived and designed the computational approach. N.A. performed statistical and machine-learning analysis. N.A. and J.S.L. analyzed the data. S.M. applied the CIBERSORT deconvolution tool. E.R., N.A. and G.Z. wrote the paper. M.H., K.F., G.B and J.S.L. helped write the paper. G.Z., B.M., D.T.F., T.M., T.T., Z.W. and R.J.S. collected the clinical and transcriptomic data under the supervision of G.Z., K.F., G.B. and M.H.

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Correspondence to Eytan Ruppin.

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The authors declare no competing interests.

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Supplementary Text and Figures

Supplementary Figures 1–10 and Supplementary Tables 1, 2, 5, 6, 8 and 11

Reporting Summary

Supplementary Table 3

Immune pathway enrichment P values and their correlation with IMPRES ratios

Supplementary Table 4

CIBERSORT analysis

Supplementary Table 7

Individual ICB response predictive power (AUC) of each of the IMPRES features

Supplementary Table 9

Melanoma patients’ identifiers and their ICB response annotations

Supplementary Table 11

IMPRES sensitivity analysis

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Auslander, N., Zhang, G., Lee, J.S. et al. Robust prediction of response to immune checkpoint blockade therapy in metastatic melanoma. Nat Med 24, 1545–1549 (2018).

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