Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Experimental microbial dysbiosis does not promote disease progression in SIV-infected macaques

Nature Medicine volume 24, pages 1313–1316 (2018)Cite this article

Subjects

Abstract

Intestinal microbial dysbiosis has been described in individuals with an HIV-1 infection and may underlie persistent inflammation in chronic infection, thereby contributing to disease progression. Herein, we induced an HIV-1-like intestinal dysbiosis in rhesus macaques (Macaca mulatta) with vancomycin treatment and assessed the contribution of dysbiosis to SIV disease progression. Dysbiotic and control animals had similar disease progression, indicating that intestinal microbial dysbiosis similar to that observed in individuals with HIV is not sufficient to accelerate untreated lentiviral disease progression.

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Rent or buy this article

Get just this article for as long as you need it

$39.95

Learn more

Prices may be subject to local taxes which are calculated during checkout

Fig. 1: Vancomycin treatment promotes dysbiosis in SIV-infected RMs.
Fig. 2: SIV disease progression is unaffected by vancomycin-induced intestinal dysbiosis.

References

  1. Ortiz, A. M. & Brenchley, J. M. Curr. Opin. HIV AIDS 13, 15–21 (2018).

    Article  CAS  Google Scholar 

  2. Brenchley, J. M., Silvestri, G. & Douek, D. C. Immunity 32, 737–742 (2010).

    Article  CAS  Google Scholar 

  3. Noguera-Julian, M. et al. EBioMedicine 5, 135–146 (2016).

    Article  Google Scholar 

  4. Policicchio, B. B., Pandrea, I. & Apetrei, C. Front. Immunol. 7, 12 (2016).

    Article  Google Scholar 

  5. Mudd, J. C. & Brenchley, J. M. J. Infect. Dis. 214 Suppl 2, S58–S66 (2016).

    Article  Google Scholar 

  6. Ivanov, I. I. et al. Cell 139, 485–498 (2009).

    Article  CAS  Google Scholar 

  7. Kelly, C. J. et al. Cell Host Microbe 17, 662–671 (2015).

    Article  CAS  Google Scholar 

  8. Day, C. L. et al. Nature 443, 350–354 (2006).

    Article  CAS  Google Scholar 

  9. Betts, M. R. et al. Blood 107, 4781–4789 (2006).

    Article  CAS  Google Scholar 

  10. McKenna, P. et al. PLoS Pathog. 4, e20 (2008).

    Article  Google Scholar 

  11. Gosmann, C. et al. Immunity 46, 29–37 (2017).

    Article  CAS  Google Scholar 

  12. d’Ettorre, G. et al. Immun. Inflamm. Dis. 5, 244–260 (2017).

    Article  Google Scholar 

  13. Vujkovic-Cvijin, I. et al. Gut Microbes 8, 440–450 (2017).

    Article  Google Scholar 

  14. Hager, C. L. & Ghannoum, M. A. Curr. Opin. HIV AIDS 13, 69–72 (2018).

    Article  CAS  Google Scholar 

  15. Ortiz, A. M. et al. Mucosal Immunol. 9, 458–467 (2016).

    Article  CAS  Google Scholar 

  16. Caporaso, J. G. et al. Nat. Methods 7, 335–336 (2010).

    Article  CAS  Google Scholar 

  17. Langille, M. G. et al. Nat. Biotechnol. 31, 814–821 (2013).

    Article  CAS  Google Scholar 

  18. Estes, J. D. et al. PLoS Pathog. 6, e1001052 (2010).

    Article  Google Scholar 

  19. Hao, X. P. et al. Nat. Commun. 6, 8020 (2015).

    Article  CAS  Google Scholar 

  20. Brenchley, J. M. et al. Blood 120, 4172–4181 (2012).

    Article  CAS  Google Scholar 

  21. Segata, N. et al. Genome Biol. 12, R60 (2011).

    Article  Google Scholar 

  22. Parks, D. H., Tyson, G. W., Hugenholtz, P. & Beiko, R. G. Bioinformatics 30, 3123–3124 (2014).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We would like to acknowledge H. Kendall, J. Swerczek, R. Herbert, and all the veterinary staff at the NIH animal center. We would like to thank M. Quiñones, J. Davis, S. Sen and G. Trinchieri, and the NIAID Microbiome Program for technical and analytical assistance. Funding for this study was provided in part by the Division of Intramural Research/NIAID/NIH, by the Oregon National Primate Research Center NIH grant award P51OD011092, and with federal funds from the National Cancer Institute, NIH, under Contract No. HHSN261200800001E. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services (DHHS), nor does the mention of trade names, commercial products, or organizations imply endorsement by the U.S. Government.

Author information

Authors and Affiliations

  1. Barrier Immunity Section, Laboratory of Viral Diseases, National Institutes of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD, USA

    Alexandra M. Ortiz, Jacob K. Flynn, Sarah R. DiNapoli, Carly E. Starke, Stephen H. Lai, MacKenzie E. Long, Ornella Sortino, Carol L. Vinton, Joseph C. Mudd & Jason M. Brenchley

  2. Mucosal Immunology Section, Laboratory of Parasitic Diseases, NIAID, NIH, Bethesda, MD, USA

    Ivan Vujkovic-Cvijin & Yasmine Belkaid

  3. AIDS and Cancer Virus Program, Frederick National Laboratory for Cancer Research, Leidos Biomedical Research, Inc., Frederick, MD, USA

    Leslie Johnston, Kathleen Busman-Sahay & Jacob D. Estes

  4. Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, OR, USA

    Kathleen Busman-Sahay & Jacob D. Estes

  5. NIAID Microbiome Program, NIH, Bethesda, MD, USA

    Yasmine Belkaid

  6. Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, OR, USA

    Jacob D. Estes

Authors
  1. Alexandra M. Ortiz
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jacob K. Flynn
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sarah R. DiNapoli
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ivan Vujkovic-Cvijin
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Carly E. Starke
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Stephen H. Lai
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. MacKenzie E. Long
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Ornella Sortino
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Carol L. Vinton
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Joseph C. Mudd
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Leslie Johnston
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Kathleen Busman-Sahay
    View author publications

    You can also search for this author in PubMed Google Scholar

  13. Yasmine Belkaid
    View author publications

    You can also search for this author in PubMed Google Scholar

  14. Jacob D. Estes
    View author publications

    You can also search for this author in PubMed Google Scholar

  15. Jason M. Brenchley
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

J.M.B. conceived the project. A.M.O. and J.M.B. designed the project and wrote the manuscript. A.M.O., J.K.F., S.R.D., C.E.C.S., S.H.L., M.E.L., O.S., C.L.V., J.C.M., L.J., K.B.-S., J.D.E. and J.M.B. performed experiments. A.M.O., J.K.F., S.R.D., I.V.-C., S.H.L., Y.B., J.D.E. and J.M.B. analyzed data.

Corresponding author

Correspondence to Jason M. Brenchley.

Ethics declarations

Competing interests

The authors declare no competing interests.

Additional information

Publisher’s note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary information

Supplementary Text and Figures

Supplementary Figures 1–8 and Supplementary Table 1

Reporting Summary

Supplementary Dataset 1

LEFSE returned results

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Ortiz, A.M., Flynn, J.K., DiNapoli, S.R. et al. Experimental microbial dysbiosis does not promote disease progression in SIV-infected macaques. Nat Med 24, 1313–1316 (2018). https://doi.org/10.1038/s41591-018-0132-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/s41591-018-0132-5

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing