Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Gene therapy

A path to efficient gene editing

Signaling by the tumor-suppressor protein p53 antagonizes CRISPR–Cas9 gene editing of human pluripotent stem cells and immortalized human retinal pigment epithelial cells.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

Fig. 1: Induction of p53 contributes to inefficient gene editing in hPSCs.

Marina Spence/Springer Nature

References

  1. 1.

    Urnov, F. D. CRISPR J. 1, 34–46 (2018).

    Article  Google Scholar 

  2. 2.

    Doudna, J. A. & Charpentier, E. Science 346, 1258096–1258096 (2014).

    Article  PubMed  CAS  Google Scholar 

  3. 3.

    Hockemeyer, D. & Jaenisch, R. Cell Stem Cell 18, 573–586 (2016).

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  4. 4.

    Ihry, R. J. et al. Nat. Med. https://doi.org/10.1038/s41591-018-0050-6 (2018).

  5. 5.

    Haapaniemi, E. et al. Nat. Med. https://doi.org/10.1038/s41591-018-0049-z (2018).

  6. 6.

    Meyers, R. M. et al. Nat. Genet. 49, 1779–1784 (2017).

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  7. 7.

    Tebas, P. et al. N. Engl. J. Med. 370, 901–910 (2014).

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  8. 8.

    Qasim, W. et al. Sci. Transl. Med. 9, eaaj2013 (2017).

    Article  PubMed  Google Scholar 

  9. 9.

    Urnov, F. D. Curr. Opin. Genet. Dev. 52, 48–56 (2018).

    Article  PubMed  CAS  Google Scholar 

  10. 10.

    Schwartz, S. D. et al. Lancet 385, 509–516 (2015).

    Article  PubMed  Google Scholar 

  11. 11.

    da Cruz, L. et al. Nat. Biotechnol. 36, 328–337 (2018).

    Article  PubMed  CAS  Google Scholar 

  12. 12.

    Dunbar, C. E. et al. Science 359, eaan4672 (2018).

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to Fyodor D. Urnov.

Ethics declarations

Competing interests

The author declares no competing interests.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Urnov, F.D. A path to efficient gene editing. Nat Med 24, 899–900 (2018). https://doi.org/10.1038/s41591-018-0110-y

Download citation

Further reading

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing