Extended Data Fig. 2: Runx3 but not CD103 are required for generation of peripherally induced CNS Trm cells. | Nature Immunology

Extended Data Fig. 2: Runx3 but not CD103 are required for generation of peripherally induced CNS Trm cells.

From: Peripherally induced brain tissue–resident memory CD8+ T cells mediate protection against CNS infection

Extended Data Fig. 2

a,b, RNA-Seq analysis of memory OT-I cells isolated from SP, IV+, or IV brains of DC-OVA-rLM-OVA prime boosted mice. Fold change of IV+ vs IV OT-I cells of corresponding genes identified in Figure 2d (a). Fold change of IV+ vs IV OT-I cells of 40 different transcription factors associated with Trm or memory CD8+ T cell responses with selected genes indicated (b). RNA samples were isolated from 5 mice pooled per group in duplicate. c, CD103KO and WT mice were DC-OVA-rLM-OVA prime-boosted and brains were harvested at a memory time point. The proportion and numbers of ova-specific CD69+ IV CD8+ T-cells are plotted. Graphs show the mean +/- standard deviation with each dot representing an individual mouse. Data are representative of 2 individual experiments with 3 mice per group. d,e, WT or CD103KO P14 cells from naïve donors were adoptively transferred into WT CD45.1 hosts prior to LCMV infection IP. At a memory time point, proportion of donor P14 cells were determined and are depicted for WT P14 cells (d) and CD103KO P14 cells (e). Graphs show the mean +/- standard deviation with each dot representing an individual mouse. Data are representative of 2 individual experiments with 5 mice per group. Statistical significance was determined by One-way ANOVA with Tukey’s multiple comparisons test across all the groups using graphpad prism with p values (top to bottom) **** p<0.0001, **** p<0.00001, and **** p<0.0001 (d) and ** p=0.0025, ** p=0.0026, and ** p=0.0033 (e). f,g, WT or Runx3KO P14 cells were adoptively transferred into WT CD45.1 hosts prior to LCMV infection IP. At a memory time point, proportion of donor P14 cells were determined and are depicted for WT P14 cells (f) and Runx3KO P14 cells (g). Graphs show the mean +/- standard deviation with each dot representing an individual mouse. Data are representative of 2 individual experiments with 5 mice per group. Statistical significance was determined by One-way ANOVA with Tukey’s multiple comparisons test across all the groups using graphpad prism with p values (top to bottom) * p=0.0159, and * p=0.0279 (f).

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