Extended Data Fig. 1: Enrichment of antigen specific CD8+ T-cells in the CNS after peripheral immunizations. | Nature Immunology

Extended Data Fig. 1: Enrichment of antigen specific CD8+ T-cells in the CNS after peripheral immunizations.

From: Peripherally induced brain tissue–resident memory CD8+ T cells mediate protection against CNS infection

Extended Data Fig. 1

a, Gating strategy used to identify CD8+ T cell populations isolated from the CNS. Representative of 3 independent experiments with 4 mice per group.b-e, The proportion of donor OT-I, P14, or OVA tetramer+ cells of live CD8+ T-cells isolated from the Spleen (SP) peripheral blood (PBL) IV+ brain (IV+) or IV brain (IV) are graphed after infection with LCMV IV, p values (top to bottom) **** p<0.0001, **** p<0.0001, ****, p<0.0001, ** p=0.0038, and * p=0.0252 (b), DC-OVA-rLM-OVA prime boost IV, p values (top to bottom) **** p<0.0001, **** p<0.0001, *** p=0.0002, *** p=0.0.0004, and *** p=0.0005 (c), DC-GP33-rLM-GP33 prime boost IV, p values (top to bottom) *** p=0.0003, *** p=0.0002, and ****p<0.0001 (d), DC-OVA-VACV-OVA IV, p values (top to bottom) ** p=0.0014, ** p=0.0019, and * p=0.0201 (e). Data represent from 2 independent experiments with 3 mice per group. Graphs show the mean +/- standard deviation with each dot representing an individual mouse. Statistical significance was determined by One-way ANOVA with Tukey’s multiple comparisons test across all the groups using graphpad prism. f, NIH Swiss Webster mice were infected with rLM-OVA IV and the proportion of live CD8+, IV CD8+ and number of IV CD8+ cells in the CNS are graphed. Data pooled from 2 independent experiments for a total of 10 mice per group. Graphs show the mean +/- standard deviation with each dot representing an individual mouse. Two-tailed unpaired students T-test were determined using graphpad prism and p values (left to right) * p=0.0458, ** p=0.0048, and * p=0.0216.

Source data

Back to article page