Extended Data Fig. 6: Peripherally induced brain resident CD8+ T-cells mediate protection against CNS infections. | Nature Immunology

Extended Data Fig. 6: Peripherally induced brain resident CD8+ T-cells mediate protection against CNS infections.

From: Peripherally induced brain tissue–resident memory CD8+ T cells mediate protection against CNS infection

Extended Data Fig. 6

a, Thy1.1 OT-I cells were transferred into mice and DC-OVA-rLM-OVA prime boosted. After memory formation, these mice and naïve controls were challenged with VSV-OVA IN. Virus titers in brains were determined at day 3 post infection. Data are combined from 2 independent experiments for a total of 10 mice per group. Graph shows the mean +/- standard deviation with each dot representing an individual mouse. Two-tailed unpaired students T-test was determined using graphpad prism where ** p=0.0021. b, Thy1.1 OT-I cells were transferred into recipient mice that were DC-OVA-rLM-OVA prime boosted. After memory formation, mice were treated with PBS or 2 μg anti-Thy1.1 Ab IP. One week after depletion these mice and naïve controls mice were challenged with VSV-OVA IC. Kaplan Meier survival curves depicted. Data from representative experiment with 6 (black), 8 (red), and 9 (blue) mice per group (top to bottom). Graphpad prism used to determine significance using Mantel-Cox test for each group comparing to the Naïve + IC challenge group with p values * p=0.014 (red), and ** p=0.0056 (blue). c,d, Thy1.1 OT-I or P14 cells were transferred into recipient mice that were DC-OVA-rLM-OVA or DC-GP33-rLM-GP33 prime boosted, respectively. After memory formation, mice were treated with PBS or 2 μg anti-Thy1.1 Ab IP. Frequency of OVA or GP33 tetramer positive cells and proportion of transgenic OT-I or P14 T-cells were determined prior to and 5 days post depletion. Representative flow plots (c) and cumulative data (d) of 2 independent experiments with 5 mice per group are shown.

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