Extended Data Fig. 5: Enhanced recall response of peripherally induced brain Trm cells is specific to CNS. | Nature Immunology

Extended Data Fig. 5: Enhanced recall response of peripherally induced brain Trm cells is specific to CNS.

From: Peripherally induced brain tissue–resident memory CD8+ T cells mediate protection against CNS infection

Extended Data Fig. 5

a,b, OT-I T-cells were transferred into recipient mice that were DC-OVA-VACV-OVA prime boosted. At a memory time point, mice were either unchallenged or challenged with rLM-OVA IC and organs were harvested 2 days later and stained for CD25 and intracellular IFN-γ directly ex vivo. Proportion of CD25+, p value **** p<0.0001 (a) and IFN-γ+, p value *** p=0.0004 (b) OT-I cells from each organ are graphed. Graphs show the mean +/- standard deviation with each dot representing an individual mouse. Data are representative of 2 independent experiments with 4 mice per group. Statistical significance was determined by two-tailed unpaired students T-test for each organ using graphpad prism. c-e, P14 T-cells were transferred into recipient mice that were immunized with LCMV IP. At a memory time point, mice were treated with PBS or 2 μg anti-Thy1.1 Ab IP. One week post depletion, mice were challenged with rLM-GP33 IC and organs were harvested 2 days later and stained for CD25 and intracellular IFN-γ directly ex vivo. Proportion of CD25+, p values (left to right) ** p=0.0099, ** p=0.0014 (c), IFN-γ+, p values (left to right) * p=0.0308, ** p=0.0069 (d) and number of IV P14 cells (e) from the brain are graphed. Graphs show the mean +/- standard deviation with each dot representing an individual mouse. Data are representative of 2 independent experiments with 3 mice per group. Two-tailed unpaired students T-test was determined using graphpad prism.

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