a-h, Thy1.1 eGFP+ OT-I T-cells were transferred into naïve mice that were DC-OVA-rLM-OVA prime boosted. Representative flow plots (a) showing proportion of OT-I cells of IV+ or IV– Thy1.1 CD8+ T-cells in each organ and cumulative proportion of OT-I cells in each organ, p values (left to right) ** p=0.0028 (b). Proportion of OT-I cells expressing CD103, p values (left to right) *** p=0.00371, * p=0.0100 (c) and CX3CR1, p values (left to right) *** p=0.0001, ** p=0.0027 (d) in IV+ vs IV– brain and meninges. gMFI of CD69, p values (left to right) *** p=0.0005, ** p=0.0013 (e), CXCR6, p values (left to right) **** p<0.0001, *** p=0.0004 (f), and CD49a, p values (left to right) **** p<0.0001, ** p=0.0025 (g) of OT-I cells from IV+ vs IV– brain and meninges are shown. SPADE analysis of OT-I cells from each organ are depicted (h). Markers used to distinguish populations include CD8α, CD11a, CD44, CD49a, CD69, CD103, CXCR6, and CX3CR1. Data are representative of 2 independent experiments with 3 mice per group. Graphs show the mean +/- standard deviation with each dot representing an individual mouse. Two-tailed unpaired l students T-test were determined using graphpad prism.