Supplementary Figure 2: Properties of anti-mouse IL-1R3 antibody (MAB-mR3) and comparison to MAB-hR3. | Nature Immunology

Supplementary Figure 2: Properties of anti-mouse IL-1R3 antibody (MAB-mR3) and comparison to MAB-hR3.

From: IL-1R3 blockade broadly attenuates the functions of six members of the IL-1 family, revealing their contribution to models of disease

Supplementary Figure 2

(a+b) MAB-mR3 (a) and MAB-hR3 (b) in a murine NIH-3T3 luciferase reporter assay (NF-κB activation) stimulated with 50pg/mL of mIL-1β, relative luciferase units (RLU) depicted. (a) IC50: 281.7 ng/mL. (c+d) MAB-mR3 (c) and MAB-hR3 (d) inhibition of IL-6 production in murine NIH-3T3 cells stimulated with hIL-1β (50 pg/mL). (c) IC50: 1276 ng/mL. (e+f) MAB-mR3 in a murine NIH-3T3 luciferase reporter assay (NF-κB activation) stimulated with (e) 1ng/mL of mIL-33 (IC50: 559.5ng/mL) or (f) 170ng/mL of mIL-36γ (IC50: 65.3ng/mL), RLU depicted. (g) MAB-mR3 kinetics analyzed using SPR (1 measurement depicted). Single-cycle kinetics were done using increasing concentrations of murine IL-1R3 (0.185nM - 15nM). Data are from one representative experiment, repeated once to confirm results. (a-d) Data made in triplicates. OD; optical density. Mean + SEM depicted.

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