(a) Plasma levels of TGFβ, IL-10, IL-4 and IFNγ were determined by ELISA and compared between relapse-free (n=25) and relapsed (n=15) BC patients. Shown are mean ± s.e.m. (b) The association between plasma level of TGFβ (p=0.08), IL-10 (p=0.79), IL-4 (p=0.36) or IFNγ (p=0.58) and signaling response in Treg II cells, respectively. Spearman correlation coefficient test. NS, not significant.