(a) Gating strategy applied in analysis of co-transfer and mixed BM chimera experiments. (b-c) Flow cytometric analysis of splenic WT or IRE1NK Ly49H+ NK cells from mixed BM chimeric mice as indicated in Supplementary Figure 2e at day 2 PI: (b)IFN-γ and (c)CD69 and intracellular Granzyme B. (d) Flow cytometric analysis of co-transferred WT or IRE1NK Ly49H+ NK cells from the spleen of Ly49H-deficient recipients at day 2 PI: pan-Akt, p-Akt, and p-S6 with quantification normalized to WT expression levels as 100%. Two tailed unpaired Student’s t-test performed on b and c. All error bars, mean with s.d. n = 6 mixed BM chimera mice in b. n = 4 mice in c. n = 3 mice in d. Data were independently repeated three times.