Supplementary Fig. 7: Bhlhe40 directly regulates gene transcription in LPMs. | Nature Immunology

Supplementary Fig. 7: Bhlhe40 directly regulates gene transcription in LPMs.

From: Bhlhe40 mediates tissue-specific control of macrophage proliferation in homeostasis and type 2 immunity

Supplementary Fig. 7

a, Gene expression microarray data were analyzed for expression of genes encoding selective regulators of LPM proliferation (Myo18a and C1q) in LPMs from Bhlhe40+/+, Bhlhe40-/-, and LysM-Cre+ Bhlhe40fl/fl mice unstimulated or treated with IL-4c. b-d, Tracings of Bhlhe40 binding, PU.1 binding, and vertebrate conservation at the Il10 (b), Ccl2 (c), and Plac8 (d) loci. e, Bhlhe40-bound, Bhlhe40-dependent genes ( ≥ 2-fold differentially expressed in Bhlhe40+/+ and Bhlhe40-/- LPMs) in LPMs from naïve mice and Bhlhe40-bound, Bhlhe40-dependent genes ( ≥ 2-fold differentially expressed in Bhlhe40+/+ and Bhlhe40-/- LPMs) in LPMs from IL-4c-treated mice, as in Fig. 8i. Underlined genes are highlighted elsewhere in this study. f-h, Tracings of Bhlhe40 binding, PU.1 binding, and vertebrate conservation at the Maf (distal) (f), Mafb (g), and Mafb (distal) (h) loci. LPM Bhlhe40 ChIP-seq data (n = 1/group), microarray data from naïve LPMs (n = 3/group), and microarray data from IL-4c-stimulated LPMs (n = 2/group) are from single separate experiments. LPM PU.1 ChIP-seq data reanalyzed from 35.

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