Supplementary Fig. 1: Characterization of Lnczc3h7a. | Nature Immunology

Supplementary Fig. 1: Characterization of Lnczc3h7a.

From: The long noncoding RNA Lnczc3h7a promotes a TRIM25-mediated RIG-I antiviral innate immune response

Supplementary Fig. 1

a, RNAi screen of the top 20 abundance lncRNAs co-precipitated with Flag-TRIM25: qRT-PCR analysis of Ifnb1 expression in peritoneal macrophages transfected with different siRNAs targeting these lncRNAs for 48 hr, then infected with VSV for 8 hr. b, 5’ and 3’ RACE assay of Lnczc3h7a in peritoneal macrophages upon VSV infection for 8 hr, using the primer of 5’RACE and 3’RACE (top). Schematic illustration of Lnczc3h7a locus on the introns of Zc3h7a and primers (labeled with arrows) used in RACE assay (below). c, Schematic illustration of different nucleotides between Lnczc3h7a and Zc3h7a intron. d, Schematic representation of PacBio long-read sequencing data shown that no transcript locus on the homologous region of Lnczc3h7a in A549 cell genome after VSV infection for 8 hr. e, Schematic illustration of primers (labeled with arrows) used in RACE assay which targeted the homologous region of 5’ end 291 bp sequence in human genome (top) and 5’ and 3’ RACE assay of equivalent Lnczc3h7a in A549 cells upon VSV infection for 8 hr (below). Data are representative of three independent experiments with n = 3 technical replicates (a), three independent experiments (b-e), each symbol represents an individual technical replicate (a) (shown as mean and s.e.m. in a), two-tailed unpaired Student’s t-test.

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