Supplementary Fig. 7: Lnczc3h7a binds to TRIM25 and RIG-I at nucleotide 308, 311 and 332 during viral infection. | Nature Immunology

Supplementary Fig. 7: Lnczc3h7a binds to TRIM25 and RIG-I at nucleotide 308, 311 and 332 during viral infection.

From: The long noncoding RNA Lnczc3h7a promotes a TRIM25-mediated RIG-I antiviral innate immune response

Supplementary Fig. 7

a, Predicted RNA secondary structure of Lnczc3h7a based on minimum free energy (MFE) (http://rna.tbi.univie.ac.at/). b, Predicted RNA secondary structure of three control transcripts based on MFE (http://rna.tbi.univie.ac.at/). c, Co-immunoprecipitation analysis of the interaction of TRIM25 with RIG-I and K63-linked ubiquitination of RIG-I in Lnczc3h7a-/- L929 cells co-transfected with Flag-TRIM25, V5-RIG-I, HA-Ub-K63 and three control RNAs or Lnczc3h7a for 24 hr, infected with VSV for 8 hr. d, Co-immunoprecipitation analysis of the interaction of TRIM25 with RIG-I and K63-linked ubiquitination of RIG-I in Lnczc3h7a-/- L929 cells co-transfected with Flag-TRIM25, V5-RIG-I, HA-Ub-K63 and different Lnczc3h7a delete mutants for 24 hr, infected with VSV for 8 hr. e, Immunofluorescence analysis of the co-localization of TRIM25 (red) and RIG-I (green)in Lnczc3h7a-/- L929 cells co-transfected with Flag-TRIM25, V5-RIG-I, HA-Ub-K63 and different Lnczc3h7a delete mutants for 24 hr, infected with VSV for 8 hr. Nuclei were stained with DAPI (blue). Scale bar, 5 μm. Data are representative of three independent experiments (c-e).

Back to article page